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首页> 外文期刊>Cell and Tissue Research >Expression of miR-206 during the initiation of mammary gland development
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Expression of miR-206 during the initiation of mammary gland development

机译：miR-206在乳腺发育启动过程中的表达

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摘要

MicroRNAs (miRNAs) are a class of small noncoding RNAs that control gene expression by targeting mRNAs and triggering either translational repression or RNA degradation. The aberrant expression of miRNAs might be involved in human diseases, including cancer. The expression of miR-206 in estrogen receptor alpha (ER-α)-positive human breast cancer tissues is well known. However, the expression and regulation of miR-206 in the developing mammary gland has not yet been studied. To understand the effects of miR-206 on mammary gland development, we have profiled gene expression in scramble-transfected and miR-206-overexpressing developing mammary buds. The genes that are potentially regulated by miR-206 in the mammary epithelium and/or mesenchyme, such as Tachykinin1 and Gata3, are known to be breast cancer markers. The expression of Wnt, which is involved in gland positioning, and of the transcription factors Tbx3 and Lef1, which are essential for mammary gland development, changes after miR-206 overexpression. Using a mammary bud in vitro culture system, we have demonstrated that miR-206 acts downstream of ER-α during mammary gland growth. Thus, miR-206 might be a novel candidate for morphogenesis during the initiation of mammary gland formation and the regulation of genes related to mammary gland development and breast cancer.
机译：MicroRNA（miRNA）是一类小的非编码RNA，它们通过靶向mRNA并触发翻译抑制或RNA降解来控制基因表达。 miRNA的异常表达可能与人类疾病（包括癌症）有关。 miR-206在雌激素受体α（ER-α）阳性的人乳腺癌组织中的表达是众所周知的。然而，尚未研究miR-206在发育中的乳腺中的表达和调控。为了了解miR-206对乳腺发育的影响，我们在混乱转染和miR-206过表达的发育中的乳芽中分析了基因表达。已知乳腺上皮和/或间充质中可能受miR-206调控的基因，例如Tachykinin1和Gata3，是乳腺癌的标志物。 miR-206过表达后，与腺体定位有关的Wnt的表达以及对乳腺发育必不可少的转录因子Tbx3和Lef1的表达发生变化。使用乳芽体外培养系统，我们已证明miR-206在乳腺生长过程中在ER-α下游起作用。因此，miR-206可能是在乳腺形成的起始阶段以及与乳腺发育和乳腺癌相关的基因调控期间形态发生的新候选者。

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