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首页> 外文期刊>Cell and Tissue Research >Repair of bone defect by using vascular bundle implantation combined with Runx II gene-transfected adipose-derived stem cells and a biodegradable matrix
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Repair of bone defect by using vascular bundle implantation combined with Runx II gene-transfected adipose-derived stem cells and a biodegradable matrix

机译：通过血管束植入结合Runx II基因转染的脂肪干细胞和可生物降解的基质修复骨缺损

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摘要

A large hurdle in orthopedics today is the difficulty of dealing with the non-union of fractured bones. We therefore evaluated the effects of runt-related transcription factor II (Runx II), a factor used to create gene-modified tissue-engineered bone, combined with vascular bundle implantation for repairing segmental bone defects. Adenovirus Runx II gene (Ad-Runx II)-modified rabbit adipose-derived stem cells (ADSCs) were seeded onto polylactic acid/polycaprolacton (PLA/PCL) scaffolds to construct gene-modified tissue-engineered bone. The following four methods were used for repair in rabbit radial-defect (1.5 cm long) models: gene-modified tissue-engineered bone with vascular bundle (Group A), gene-modified tissue-engineered bone (Group B), non-gene-modified tissue-engineered bone with vascular bundle (Group C), and PLA/PCL scaffolds only (Group D). X-ray, histological examination, biomechanics analysis, and micro-angiography were conducted 4, 8, and 12 weeks later to determine angiogenesis and osteogenesis. The volume and speed of production of newly formed bones in Group A were significantly superior to those in other groups, and de-novo vascular network circulation from the vessel bundle through newly formed bone tissue was observed, with the defect being completely repaired. Group B showed a slightly better effect in terms of speed and quality of bone formation than Group C, whereas the bone defect in Group D was replaced by fibrous tissue. The maximal anti-bending strength in Group A was significantly higher than that in the other groups. Runx II gene therapy combined with vascular bundle implantation thus displays excellent abilities for osteoinduction and vascularization and is a promising method for the treatment of bone non-union and defect.
机译：今天，整形外科的一大障碍是应对骨折骨不愈合的困难。因此，我们评估了矮子相关转录因子II（Runx II）的作用，该因子用于创建基因修饰的组织工程骨，并与血管束植入相结合以修复节段性骨缺损。将腺病毒Runx II基因（Ad-Runx II）修饰的兔脂肪来源的干细胞（ADSC）接种到聚乳酸/聚己内酰胺（PLA / PCL）支架上，以构建基因修饰的组织工程骨。以下四种方法用于修复兔radial骨畸形（1.5 cm长）模型：带血管束的基因修饰的组织工程骨（A组），基因修饰的组织工程骨（B组），非基因血管束的改良组织工程骨（C组），仅PLA / PCL支架（D组）。在第4、8和12周后进行X射线，组织学检查，生物力学分析和微血管造影，以确定血管生成和成骨作用。 A组中新形成的骨头的体积和生产速度明显优于其他组，并且观察到从血管束到新形成的骨组织的新生血管网络循环，并完全修复了缺损。 B组在骨形成的速度和质量方面显示出比C组更好的效果，而D组的骨缺损被纤维组织所替代。 A组的最大抗弯曲强度明显高于其他组。 Runx II基因疗法与血管束植入术的结合因此显示出优异的骨诱导和血管形成能力，是治疗骨不连和缺损的有前途的方法。

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