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Effects of PTHrP on chondrocytes of sika deer antler

机译：PTHrP对梅花鹿鹿角软骨细胞的影响

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摘要

Parathyroid-hormone-related peptide (PTHrP) is an important regulator of chondrocyte differentiation in growth plates but little is known about its role in deer antler cartilage. The aim of the present study was to use the deer antler as a model to determine the possible role of PTHrP in regulating chondrocyte differentiation of deer antler. PTHrP and its receptor PTH1R mRNA were highly expressed in the perichondrium and cartilage of sika deer antler, as shown by in situ hybridization. Chondrocytes of deer antler were identified by toluidine blue staining of glycosaminoglycan and immunocytochemical staining of type II collagen (Col II). Treatment with PTHrP (1-34) reduced the expression of prehypertrophic chondrocyte marker Col IX and hypertrophic chondrocyte marker Col X. In order to confirm the mechanism of action of PTHrP, we initially examined the expression of cyclin D1, Bcl-2 and runt-related transcription factor 2 (Runx2) in sika deer antler by in situ hybridization and found that cyclin D1, Runx2 and Bcl-2 mRNA were also expressed in antler chondrocytes. Exogenous PTHrP induced the expression of cyclin D1 and Bcl-2 mRNA by various signalling pathways, whereas it inhibited Runx2 expression through PKA, p38MAPK, MEK and PI3K signalling pathways. Thus, PTHrP might promote the proliferation of antler chondrocytes and prevent their differentiation; it might furthermore influence the growth and development of sika deer antler.
机译：甲状旁腺激素相关肽（PTHrP）是生长板中软骨细胞分化的重要调节剂，但对其在鹿角软骨中的作用知之甚少。本研究的目的是使用鹿茸作为模型来确定PTHrP在调节鹿茸软骨细胞分化中的可能作用。原位杂交表明，PTHrP及其受体PTH1R mRNA在梅花鹿角的软骨膜和软骨中高表达。鹿角软骨细胞通过糖胺聚糖的甲苯胺蓝染色和II型胶原蛋白（Col II）的免疫细胞化学染色来鉴定。用PTHrP（1-34）处理可减少肥大前软骨细胞标记物Col IX和肥大性软骨细胞标记物Col X的表达。通过原位杂交检测梅花鹿鹿角中的转录因子2（Runx2），发现鹿角软骨细胞中也表达了cyclin D1，Runx2和Bcl-2 mRNA。外源性PTHrP通过多种信号途径诱导细胞周期蛋白D1和Bcl-2 mRNA的表达，而通过PKA，p38MAPK，MEK和PI3K信号途径抑制Runx2表达。因此，PTHrP可能促进鹿角软骨细胞的增殖并阻止其分化。它可能进一步影响梅花鹿茸的生长发育。

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