...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Inhibition of focal adhesion kinase expression correlates with changes in the cytoskeleton but not apoptosis in primary cultures of chick embryo cells.
【24h】

Inhibition of focal adhesion kinase expression correlates with changes in the cytoskeleton but not apoptosis in primary cultures of chick embryo cells.

机译：粘着斑激酶表达的抑制与鸡胚细胞原代培养中细胞骨架的变化有关，但与细胞凋亡无关。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cell adhesion to the extracellular matrix through integrin receptors can activate signaling cascades within the cell. Focal adhesion kinase (FAK) is a protein tyrosine kinase activated by integrin adhesion. The role of FAK within the cell is not clear, although evidence suggests roles in cell motility or the regulation of adhesion-dependent cell survival. We have treated primary cultures of chick embryo cells with antisense oligonucleotides to FAK to reduce the level of FAK protein expression. Levels of the related protein, proline-rich tyrosine kinase 2 (Pyk2) and the FAK substrate paxillin, were unaffected by the addition of oligonucleotides, whereas FAK expression was reduced by 70%. Levels of apoptotic cell death did not significantly increase after the addition of oligonucleotides. However, there was a change in the distribution of focal adhesion sites from a uniformly distributed pattern to a mainly peripheral pattern. This was accompanied by a loss of stress fibers and an increase in the peripheral actin cytoskeleton, as the cells became rounded. These results suggest that in these early embryonic cells, FAK expression regulates the arrangement of focal adhesions and the cytoskeleton that result in a motile phenotype, but that FAK does not appear to regulate apoptosis. Copyright 2001 Academic Press.
机译：通过整联蛋白受体将细胞粘附到细胞外基质上可以激活细胞内的信号级联反应。局灶性粘附激酶（FAK）是一种由整合素粘附激活的蛋白酪氨酸激酶。 FAK在细胞内的作用尚不清楚，尽管有证据表明FAK在细胞运动或粘附依赖性细胞存活的调控中起作用。我们已经用FAK的反义寡核苷酸处理了鸡胚细胞的原代培养物，以降低FAK蛋白表达的水平。相关蛋白，富含脯氨酸的酪氨酸激酶2（Pyk2）和FAK底物paxillin的水平不受寡核苷酸添加的影响，而FAK表达降低了70％。加入寡核苷酸后，凋亡细胞死亡水平没有显着增加。但是，粘着部位的分布从均匀分布的图案变为主要为外周的图案。随着细胞的变圆，伴随着应力纤维的丢失和周围肌动蛋白细胞骨架的增加。这些结果表明，在这些早期胚胎细胞中，FAK的表达调节粘着斑和细胞骨架的排列，从而导致运动型，但FAK似乎没有调节细胞凋亡。版权所有2001，学术出版社。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号