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首页> 外文期刊>Cell biology international. >Early membrane depressions in hepatocytes cultured on Primaria supports.
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Early membrane depressions in hepatocytes cultured on Primaria supports.

机译：在Primaria支持物上培养的肝细胞中早期的膜凹陷。

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摘要

The topography of spreading hepatocytes on positively charged Primaria plates was examined using scanning electron microscopy. The cells acquired a uniform morphology within 2 h. The spreading was rapid and the surface of the cells showed early prominent depressions or dips. The hepatocytes had either one or two of these structures which corresponded to the frequency of mononuclear and binuclear cells, respectively. The nuclear origin of the dips was strengthened after 6 h. They contained solid structures, whose number, size and shape were the same as nucleoli. The membrane dipping was independent of cell density and took place under conditions where phenotypic changes can occur. Kidney proximal tubule cells had no dips. Co-cultures of hepatocytes and kidney proximal tubule cells showed that the cell types behave differently.
机译：使用扫描电子显微镜检查在带正电荷的Primaria板上散布的肝细胞的形貌。细胞在2小时内获得了均匀的形态。扩散迅速并且细胞表面显示出早期显着的凹陷或凹陷。肝细胞具有这些结构中的一种或两种，分别对应于单核细胞和双核细胞的频率。 6小时后浸入的核起源得到加强。它们包含固体结构，其数量，大小和形状与核仁相同。膜浸没与细胞密度无关，发生在可能发生表型变化的条件下。肾脏近端小管细胞无浸染。肝细胞和肾近端小管细胞的共培养表明，细胞类型的行为不同。

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