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首页> 外文期刊>Cell biology international. >Giant vacuoles observed in Dictyostelium discoideum.
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Giant vacuoles observed in Dictyostelium discoideum.

机译：在盘基网柄菌中观察到巨大的液泡。

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Large intracellular vacuoles, >4 microm in diameter and either round or oval-shaped, were observed infrequently in Dictyostelium discoideum amoebae of axenically-grown strain AX2 (only 1 in 10(6)-10(8)cells). These previously unreported single or multiple 'giant' vacuoles were more common, however, in newly germinated KAX3 cells (0.55% of the population) and AT-K(neg), a strain that lacks an esterase (0.47% of the population). A vacuolar H(+)-ATPase was enriched in their membranes of intracellular giant vacuoles, indicating that the vacuoles were related possibly to both endosomes and the contractile vacuole compartment. When monitored over time, giant vacuoles protruded from, and retracted back into cells under hyperosmotic conditions, suggesting an osmoregulatory role for these vacuoles. Some of the intracellular and protruded giant vacuoles harbored a fluid-phase marker, fluorescein-labeled dextran, implying a pinocytotic origin for the vacuoles. Copyright 2001 Academic Press.
机译：在轴突生长的菌株AX2的Discyostelium discoideum amoebae中，很少见到直径> 4微米的圆形或椭圆形的大细胞内液泡（仅10（6）-10（8）个细胞中有1个）。这些以前未报告的单个或多个“巨人”液泡在新发芽的KAX3细胞（占种群的0.55％）和AT-K（阴性）菌株中缺乏酯酶（占种群的0.47％）更为普遍。液泡H（+）-ATPase富集在其胞内巨液泡膜中，表明液泡可能与内体和收缩液泡区室有关。当随着时间的推移进行监测时，在高渗条件下，巨大的液泡从细胞中突出并缩回细胞中，表明这些液泡具有渗透调节作用。一些细胞内和突出的巨大液泡带有一个液相标记，荧光素标记的葡聚糖，暗示液泡的胞吐起源。版权所有2001，学术出版社。

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