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首页> 外文期刊>Cell biology international. >INVOLVEMENT OF GLUTATHIONE AND GLUTATHIONE-RELATED ENZYMES IN THE PROTECTION OF NORMAL AND TRISOMIC HUMAN FIBROBLASTS AGAINST DAUNORUBICIN.
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INVOLVEMENT OF GLUTATHIONE AND GLUTATHIONE-RELATED ENZYMES IN THE PROTECTION OF NORMAL AND TRISOMIC HUMAN FIBROBLASTS AGAINST DAUNORUBICIN.

机译：谷胱甘肽和谷胱甘肽相关酶在预防道诺霉素中的正常和三体性人类成纤维细胞中的参与。

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We measured the glutathione content, and the activity of glutathione-related enzymes and DT-diaphorase in cultured normal (cell line: S-126) and trisomic (cell lines: S-158, S-240) human fibroblasts exposed to daunorubicin (DNR). Determination of reduced and total glutathione levels, and measurement of the activity of glutathione peroxidase, glutathione reductase, glutathione-S-transferase and DT-diaphorase were performed spectrophotometrically. Human fibroblasts were exposed to 4 &mgr;m DNR for 2 h, and the cells placed in drug-free medium for 6, 12, 24, 48, and 72 h. Cellular levels of GSH and total glutathione decreased following exposure to DNR. However, the ratio of GSH to total glutathione returned to control levels only in trisomic cells. These changes were concomitant with increasing glutathione-S-transferase and glutathione reductase activities. DNR also significantly increased the activity of Se-independent peroxidase and DT-diaphorase in trisomic fibroblasts. Marked increases in the activity of Se-dependent peroxidase and DT-diaphorase alone were seen in normal cells. The results provide the first evidence that DNR can induce alterations in the level of glutathione and glutathione-dependent enzymes in trisomic fibroblasts as compared to normal cells, which may provide additional protection against daunorubicin-induced oxidative stress in trisomic fibroblasts. Copyright 2002 Published by Elsevier Science Ltd.
机译：我们测量了暴露于柔红霉素（DNR）的正常人（细胞系：S-126）和三体（细胞系：S-158，S-240）人成纤维细胞中的谷胱甘肽含量以及谷胱甘肽相关酶和DT-黄递酶的活性）。分光光度法测定降低的谷胱甘肽和总谷胱甘肽水平，以及测量谷胱甘肽过氧化物酶，谷胱甘肽还原酶，谷胱甘肽-S-转移酶和DT-黄递酶的活性。将人成纤维细胞暴露于4μmDNR 2 h，然后将细胞置于无毒培养基中6、12、24、48和72 h。接触DNR后，细胞内GSH和总谷胱甘肽水平降低。但是，仅在三体细胞中，GSH与总谷胱甘肽的比例才恢复到对照水平。这些变化与增加的谷胱甘肽-S-转移酶和谷胱甘肽还原酶活性同时发生。 DNR还显着增加了三体性成纤维细胞中Se无关的过氧化物酶和DT-黄递酶的活性。在正常细胞中，仅硒依赖性过氧化物酶和DT-黄递酶的活性显着增加。该结果提供了第一个证据，即与正常细胞相比，DNR可以诱导三体成纤维细胞中谷胱甘肽和谷胱甘肽依赖性酶水平的改变，这可以为三柔子成纤维细胞中柔红霉素诱导的氧化应激提供额外的保护。版权所有2002，由Elsevier Science Ltd发布。

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