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首页> 外文期刊>Cell biology international. >Non-invasive microscopy of tip-growing root hairs as a tool for study of dynamic and cytoskeleton-based vesicle trafficking.
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Non-invasive microscopy of tip-growing root hairs as a tool for study of dynamic and cytoskeleton-based vesicle trafficking.

机译：尖端生长的根毛的无创显微镜作为研究动态和基于细胞骨架的囊泡运输的工具。

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摘要

The techniques of live cell imaging by electronic light microscopy and confocal microscopy were used to analyse the cytoarchitecture, organelle dynamics and membrane trafficking in living root hairs of Arabidopsis thaliana and Medicago sativa. We focused on the motility of vesicles in the tip of growing root hairs, the internalisation of plasma membrane by endocytosis and the fate of endocytic compartments. Vesicles as well as their trafficking to and contact with the plasma membrane were visualised in the tip of root hairs. We showed rapid endocytosis using a pulse treatment with lipophillic FM dyes in growing root hairs. Endocytosis was active at the very tip and labelled endocytic membranes progressed further down the endocytic network through dynamic compartments merging with the vacuole by their fusion with highly dynamic tonoplast.
机译：利用电子光学显微镜和共聚焦显微镜对活细胞成像的技术用于分析拟南芥和紫花苜蓿活根毛的细胞结构，细胞器动力学和膜运输。我们专注于生长的根毛尖端的囊泡的运动性，内吞作用导致质膜的内在化和内吞隔室的命运。囊泡以及它们向质膜的运输和接触都在根毛的尖端可见。我们显示了在生长的根毛中使用亲脂性FM染料进行脉冲处理后的快速内吞作用。内吞作用非常活跃，标记的内吞膜通过与高动态液泡膜融合的动态区室与液泡合并，进一步沿着内吞网络向下发展。

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