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首页> 外文期刊>Cell biology international. >Effect of silicon dioxide on expression of poly (ADP-ribose) polymerase mRNA and protein.
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Effect of silicon dioxide on expression of poly (ADP-ribose) polymerase mRNA and protein.

机译：二氧化硅对聚（ADP-核糖）聚合酶mRNA和蛋白质表达的影响。

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Silicon dioxide induces acute injury and chronic pulmonary fibrosis. International Agency for Research on Cancer (IARC) listed it as a human carcinogen in 1996. However, the molecular mechanisms to induce cancer are not understood yet. The content of poly (ADP-ribose) polymerases (PARP) mRNA and protein in Hela cells treated with concentrations of silicon dioxide up to 400microg/ml was determined by real-time fluorogenetic quantitative PCR (RQ-PCR) and immunofluorescence assay, respectively. MTT assay was used to determine cell viability. The results showed that viability at 400microg/ml silica was significantly decreased but not at lower concentrations. The protein content of gamma-H2AX in silica-treated group was significantly higher than the controls. The PARP mRNA and protein levels were significantly reduced with a dose response manner from the lowest silicon dioxide level. Our findings suggested that silicon dioxide increased the expression of gamma-H2AX and inhibited the expression of PARP mRNA and protein in Hela cells.
机译：二氧化硅会引起急性损伤和慢性肺纤维化。国际癌症研究机构（IARC）于1996年将其列为人类致癌物。但是，诱导癌症的分子机制尚不清楚。分别通过实时荧光定量PCR（RQ-PCR）和免疫荧光测定法测定了浓度高达400μg/ ml的二氧化硅处理的Hela细胞中聚（ADP-核糖）聚合酶（PARP）mRNA和蛋白质的含量。使用MTT测定法测定细胞活力。结果表明，在400microg / ml硅胶上的活力显着降低，但在较低浓度下没有降低。硅胶治疗组中γ-H2AX的蛋白质含量显着高于对照组。 PARP mRNA和蛋白水平从最低的二氧化硅水平开始以剂量反应的方式显着降低。我们的发现表明，二氧化硅可增加Hela细胞中γ-H2AX的表达，并抑制PARP mRNA和蛋白的表达。

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