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首页> 外文期刊>Cell biology international. >Establishing in vitro Zinnia elegans cell suspension culture with high tracheary element differentiation.
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Establishing in vitro Zinnia elegans cell suspension culture with high tracheary element differentiation.

机译：建立具有高气管因子分化能力的体外百日草线虫细胞悬液培养。

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The Zinnia elegans mesophyll cell culture is a useful system for xylogenesis studies. The system is associated with highly synchronous tracheary element (TE) differentiation, making it more suitable for molecular studies requiring larger amounts of molecular isolates, such as mRNA and proteins and for studying cellulose synthesis. There is, however, the problem of non-uniformity and significant variations in the yields of TEs (%TE). One possible cause for this variability in the %TE could be the lack of a standardized experimental protocol in various research laboratories for establishing the Zinnia culture. Mesophyll cells isolated from the first true leaves of Z. elegans var Envy seedlings of approximately 14 days old were cultured in vitro and differentiated into TEs. The xylogenic culture medium was supplied with 1mg/l each of benzylaminopurine (BA) and alpha-naphthalene acetic acid (NAA). Application of this improved culture method resulted in stable and reproducible amounts of TE as high as 76% in the Zinnia culture. The increase was mainly due to conditioning of the mesophyll cell culture and adjustments of the phytohormonal balance in the cultures. Also, certain biochemical and cytological methods have been shown to reliably monitor progress of TE differentiation. We conclude that, with the adoption of current improvement in the xylogenic Z. elegans culture, higher amounts of tracheary elements can be produced. This successful outcome raises the potential of the Zinnia system as a suitable model for cellulose and xylogenesis research.
机译：百日草属叶肉细胞培养物是用于木发生研究的有用系统。该系统与高度同步的气管元件（TE）分化相关，使其更适合于需要大量分子分离物（例如mRNA和蛋白质）的分子研究以及纤维素合成的研究。然而，存在不均匀和TE的产率（％TE）显着变化的问题。 ％TE发生这种变化的一个可能原因可能是各种研究实验室缺乏建立百日草文化的标准化实验方案。从约14天大的线虫Z. elegans var Envy幼苗的第一片真叶中分离出的叶肉细胞在体外培养，并分化为TEs。向生木培养基中分别提供1mg / l的苄氨基嘌呤（BA）和α-萘乙酸（NAA）。这种改进的培养方法的应用导致百日草培养物中稳定且可重现的TE量高达76％。增加主要是由于调节叶肉细胞培养物和调节培养物中植物激素平衡。同样，某些生化和细胞学方法已经显示出可以可靠地监测TE分化的进程。我们得出的结论是，随着对木本生线虫线虫培养物的当前改进的采用，可以产生更多的气管元素。这一成功的结果提高了百日草系统作为纤维素和木糖异生研究的合适模型的潜力。

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