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首页> 外文期刊>Cell biology international. >Experimental reconsideration of the utility of serum starvation as a method for synchronizing mammalian cells.
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Experimental reconsideration of the utility of serum starvation as a method for synchronizing mammalian cells.

机译：对血清饥饿作为同步哺乳动物细胞的方法进行实验性的重新思考。

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摘要

Accurate cell-size determinations support the prediction that serum starvation and related whole-culture methods cannot synchronize cells. Theoretical considerations predict that whole-culture methods of synchronization cannot synchronize cells. Upon serum starvation, the fraction of cells with a G1-phase amount of DNA increased, but the cell-size distribution is not narrowed. In true synchronization, the cell-size distribution should be narrower than the cell-size distribution of the original culture. In contrast, cells produced by a selective (i.e. non-whole-culture) method have a specific DNA content, a narrow size distribution, and divide synchronously. The general theory leading to the conclusion that whole-culture methods for synchronization do not work implies that one can generalize these serum-starvation results to other cell lines and other whole-culture methods, to conclude that these methods do not synchronize cells.
机译：准确的细胞大小测定结果支持以下预测：血清饥饿和相关的全培养方法无法使细胞同步。从理论上考虑，同步的全培养方法无法同步细胞。血清饥饿时，具有G1相DNA量的细胞比例增加，但细胞大小分布没有变窄。在真正的同步中，细胞大小分布应比原始培养物的细胞大小分布窄。相反，通过选择性（即非全培养）方法产生的细胞具有特定的DNA含量，窄的尺寸分布并同步分裂。导致得出结论的全培养同步方法不起作用的一般理论意味着人们可以将这些血清饥饿的结果推广到其他细胞系和其他全培养方法，以得出这些方法不能使细胞同步的结论。

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