• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Evidence of a role for S. cerevisiae alpha-arrestin Art1 (Ldb19) in mating projection and zygote formations
【24h】

Evidence of a role for S. cerevisiae alpha-arrestin Art1 (Ldb19) in mating projection and zygote formations

机译：酿酒酵母α-arrestinArt1（Ldb19）在交配投射和合子形成中的作用的证据

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The discovery of arrestin-mediated biased signalling mechanisms for mammalian G-protein coupled receptors (GPCRs) has revolutionized the field over the last decade. Now, with the recent demonstration of a role for alpha-arrestins in internalization of the yeast pheromone GPCR, Ste2p, the possibility of arrestin-mediated alternate GPCR functionalities in yeast also follows. Here, the effects of knockout and complementation of yeast a- arrestin expression during mating are reported. Although minor effects on classical pheromone-related signalling are noted for a few arrestins, much stronger effects were observed downstream of cell cycle arrest, in particular linking Ldb19 (Art1) to mediation of zygote formation. Subsequent phenotypic observations linked this activity to more pronounced projection formation in an Art1 complemented noncuple alpha-arrestin knockout line, compared to the knockout-line alone, or either of the Art3 or Art6 complemented lines. Together with the observation of ligand-stimulated localization of Art-GFP to the mating projection, a possible role for this arrestin-like protein in projection formation is supported. While leaving the full mechanism of alternate Art1 functionality to be elucidated, together these findings implicate Art1 in selective regulation of mating events downstream of receptor internalization and cell cycle arrest, leading to schmoo, and ultimately zygote formation.
机译：在过去的十年中，针对哺乳动物G蛋白偶联受体（GPCR）的抑制蛋白介导的偏向信号转导机制的发现彻底改变了这一领域。现在，随着最近证明α-arrestins在酵母信息素GPCR Ste2p内在化中的作用，也随之出现了抑制蛋白在酵母中介导的交替GPCR功能的可能性。在此，报道了交配过程中酵母α-抑制素表达的敲除和互补作用。尽管注意到一些抑制蛋白对经典信息素相关信号的影响很小，但在细胞周期抑制的下游观察到了更强的作用，特别是将Ldb19（Art1）与合子形成的介导联系起来。随后的表型观察结果表明，与单独的基因敲除谱系或Art3或Art6补充谱系相比，Art1互补的非杯形α-arrestin基因敲除系中该活性与更明显的突起形成有关。与观察到配体刺激的Art-GFP定位到交配的投影相结合，支持了这种抑制蛋白样蛋白在投影形成中的可能作用。尽管尚待阐明替代Art1功能的完整机理，但这些发现共同暗示Art1选择性调节受体内化和细胞周期停滞下游的交配事件，导致schmoo并最终形成合子。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号