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首页> 外文期刊>Cell biology international. >DIGITAL IMAGE ANALYSIS OF CHROMATIN FIBRE PHENOTYPE AFTER 'IN SITU' DIGESTION WITH RESTRICTION ENDONUCLEASES
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DIGITAL IMAGE ANALYSIS OF CHROMATIN FIBRE PHENOTYPE AFTER 'IN SITU' DIGESTION WITH RESTRICTION ENDONUCLEASES

机译：限制性内切酶“原位”消化后染色质纤维表型的数字图像分析

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摘要

Restriction Endonucleases (REs) may recognize, cleave and remove DNA from fixed chromatin producing specific chromosome banding patterns. However, the modifications produced in the chromatin fibre are not easy to evaluate and compare. The aim of the present investigation was to visualize differences resulting in the texture of the chromatin fibre from metaphase chromosomes after each digestion using digital image analysis (DIA) facilities. To this purpose, metaphase chromosomes derived from a L-929 mouse cell line were digested with different REs (AM, HpaII and HaeIII). Since light microscopy does not permit the observation of the chromatin fibre, DIA was performed on digitalized images of metaphase chromosomes under electron microscopy. The application of a LUT (Look Up Table) within the DIA software assigns a colour to each grey level of a digital image. The results obtained using a particular LUT, which permits the discrimination of specific chromatin fibre phenotypes resulting from each digestion, are reported and compared with those obtained under the light microscope.
机译：限制性核酸内切酶（REs）可以从固定的染色质中识别，切割并去除DNA，从而产生特定的染色体条带模式。但是，染色质纤维中产生的修饰不容易评估和比较。本研究的目的是使用数字图像分析（DIA）工具在每次消化后可视化导致中期染色体染色质纤维质地的差异。为此，用不同的RE（AM，HpaII和HaeIII）消化了来自L-929小鼠细胞系的中期染色体。由于光学显微镜不允许观察染色质纤维，因此在电子显微镜下对中期染色体的数字化图像进行了DIA。 DIA软件中LUT（查找表）的应用将颜色分配给数字图像的每个灰度级。报告了使用特定LUT获得的结果，该结果可以区分每次消化产生的特定染色质纤维表型，并将其与光学显微镜下获得的结果进行比较。

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