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首页> 外文期刊>Cell biology international. >Cellular organisation and differentiation of organelles in pre-meiotic rice anthers.
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Cellular organisation and differentiation of organelles in pre-meiotic rice anthers.

机译：减数分裂前花药中细胞器的细胞组织和分化。

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摘要

Pre-meiotic cellular organisation of rice anthers has a great significance in pollen formation. We have used a combination of confocal laser and transmission electron microscopy (TEM) to characterise and differentiate organelles in pre-meiotic rice anthers. Along with the characteristic organelles in the cytoplasm the epidermal cells of the pre-meiotic rice anther are coated on their outer surface by a conspicuous bi-lamellate cuticle. Chloroplasts of the endothecium contain immature grana, thylakoids and also starch granules. These plastids clearly contain photosynthetic pigments as shown by autofluorescence in confocal microscope studies. Both confocal and TEM studies reveal clusters of mitochondria in the middle layer. The tapetum contains electron opaque ribosomes, bundles of mitochondria and plastids. The nuclei of the tapetum occupy a large volume of the cytoplasm indicating the onset of mitotic prophase. Intense Rhodamine 123 staining reveals that a major portion of the structurally indistinguishable organelles that were seen throughout the densely ribosomic cytoplasm of sporogenous cells are mitochondria.
机译：水稻花药减数分裂前的细胞组织在花粉形成中具有重要意义。我们已经使用共聚焦激光和透射电子显微镜（TEM）的组合来表征和区分减数分裂前水稻花药中的细胞器。减数分裂前的水稻花药的表皮细胞与胞浆中的特征性细胞器一起被明显的双层状表皮覆盖。内皮的叶绿体含有未成熟的颗粒，类囊体以及淀粉颗粒。如共聚焦显微镜研究中的自发荧光所示，这些质体显然含有光合色素。共聚焦和透射电镜研究均显示中层线粒体簇。绒毡层包含电子不透明核糖体，线粒体束和质体。绒毡层的核占据大量的细胞质，表明有丝分裂前期的开始。强烈的若丹明123染色显示，在整个孢原细胞的密集核糖体细胞质中可见的大部分结构上无法区分的细胞器是线粒体。

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