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首页> 外文期刊>Cell biology international. >Decrease in p53 protein in cultured cardinal ligament fibroblasts from patients with prolapsus uteri.
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Decrease in p53 protein in cultured cardinal ligament fibroblasts from patients with prolapsus uteri.

机译：患有子宫垂体的患者培养的心脏韧带成纤维细胞中p53蛋白的减少。

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The authors examined the growth response of cardinal ligamental fibroblasts derived from patients with prolapsus uteri (HPLiF) and compared it with the response of those from control subjects (HCLiF). The growth rate during the logarithmic growth phase was not different between HPLiF and HCLiF, while the cell density at confluence (saturation density) was significantly higher in HPLiF than in HCLiF. When added alone, platelet-derived growth factor (PDGF), insulin-like growth factor-I (IGF-I), and epidermal growth factor (EGF) produced minimal effects on DNA synthesis in HCLiF. The simultaneous addition of PDGF, IGF-I and EGF synergistically stimulated the DNA synthesis. In contrast, PDGF alone was able to initiate DNA synthesis in HPLiF. The combination of PDGF, IGF-I, and EGF significantly stimulated the DNA synthesis of HPLiF compared with HCLiF. p53 protein and p53 gene transcripts decreased by 50% in HPLiF. The anti-WAF1 antibody reacted intensely with a 21-kDa protein in the homogenates of control fibroblasts, while the immunoreactive band in prolapsus fibroblasts was clearly reduced. These results indicate that the higher proliferative activity at near confluency in prolapsus fibroblasts may result from the decreased expression of p53 protein and p53 mRNA followed by the decrease in p21 protein. Furthermore, the failure of cells to enter quiescence may lead to a decrease in the synthesis and deposition of elastin and thus may contribute to the loss of supportive function in uterine connective tissues. Copyright 1998 Academic Press.
机译：作者检查了来自子宫无垂体（HPLiF）患者的基本韧带成纤维细胞的生长反应，并将其与对照对象（HCLiF）的反应进行了比较。 HPLiF和HCLiF之间的对数生长期的增长率没有差异，而HPLiF中汇合时的细胞密度（饱和密度）显着高于HCLiF。当单独添加时，血小板衍生生长因子（PDGF），胰岛素样生长因子-I（IGF-1）和表皮生长因子（EGF）对HCLiF中的DNA合成影响很小。同时加入PDGF，IGF-1和EGF协同刺激DNA合成。相反，仅PDGF就能启动HPLiF中的DNA合成。与HCLiF相比，PDGF，IGF-I和EGF的组合显着刺激了HPLiF的DNA合成。在HPLiF中，p53蛋白和p53基因转录物降低了50％。在对照成纤维细胞的匀浆中，抗WAF1抗体与21-kDa蛋白强烈反应，而在成年成纤维细胞中的免疫反应带明显减少。这些结果表明，在接近汇合的成纤维细胞中较高的增殖活性可能是由于p53蛋白和p53 mRNA的表达降低，随后是p21蛋白的降低。此外，细胞不能进入静止状态可能导致弹性蛋白的合成和沉积减少，因此可能导致子宫结缔组织中支持功能的丧失。版权所有1998学术出版社。

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