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首页> 外文期刊>Cell biology international. >Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations.
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Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations.

机译：体外暴露于不同锌浓度后牛卵丘细胞凋亡和DNA损伤的分析。

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The purpose of this study was to investigate the effect of Zn (zinc) concentration on CCs (cumulus cells) during in vitro maturation. For this purpose, DNA integrity of CCs by addition of different Zn concentrations [0 (control); 0.7 mug/ml (Zn1); 1.1 mug/ml (Zn2) and 1.5 mug/ml (Zn3)] to the culture medium was evaluated by comet assay. In addition, early apoptosis was analysed by annexin staining assay. CCs treated with Zn showed a significant decrease in the DNA damage in a dose-dependent manner. Comet assay analysed for TM (tail moment) was significantly higher in cells cultured without Zn (control, P<0.01) with respect to cells treated with Zn (control: 5.24+/-16.05; Zn1: 1.13+/-5.31; Zn2: 0.10+/-0.36; Zn3: 0.017+/-0.06). All treatments were statistically different from the control (P = 0.014 for Zn1; P<0.01 for Zn2 and Zn3). The frequency of apoptotic cells was higher in the control group (control: 0.142+/-0.07; Zn1: 0.109+/-0.0328; Zn2:0.102+/-0.013; Zn3: 0.0577+/-0.019). Statistical differences were found between control and Zn1 (P = 0.0308), control and Zn2 (P = 0.0077), control and Zn3 (P<0.0001), Zn1 and Zn3 (P<0.001) and Zn2 and Zn3 (P = 0.0004). No differences were found between Zn1 and Zn2. In conclusion, low Zn concentrations increase DNA damage and apoptosis in CCs cultured in vitro. However, adequate Zn concentrations 'protect' the integrity of DNA molecule and diminish the percentage of apoptotic CC.
机译：这项研究的目的是研究体外成熟过程中Zn（锌）浓度对CC（卵丘细胞）的影响。为此目的，通过添加不同的锌浓度[0（对照）; 0.7杯/毫升（Zn1）;通过彗星试验评估对培养基的1.1杯/毫升（Zn 2）和1.5杯/毫升（Zn 3）。另外，通过膜联蛋白染色测定法分析了早期凋亡。用锌处理的CCs显示出DNA损伤的剂量依赖性显着降低。相对于用Zn处理的细胞（对照：5.24 +/- 16.05; Zn1：1.13 +/- 5.31; Zn2： 0.10 +/- 0.36; Zn3：0.017 +/- 0.06）。所有处理均在统计学上与对照不同（Zn1为P = 0.014; Zn2和Zn3为P <0.01）。对照组中凋亡细胞的频率更高（对照：0.142 +/- 0.07; Zn1：0.109 +/- 0.0328; Zn2：0.102 +/- 0.013; Zn3：0.0577 +/- 0.019）。在对照和Zn1（P = 0.0308），对照和Zn2（P = 0.0077），对照和Zn3（P <0.0001），Zn1和Zn3（P <0.001）以及Zn2和Zn3（P = 0.0004）之间发现统计学差异。 Zn1和Zn2之间没有发现差异。总之，低锌浓度会增加体外培养CC中DNA的损伤和细胞凋亡。但是，适当的锌浓度可以“保护” DNA分子的完整性，并减少凋亡CC的百分比。

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