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首页> 外文期刊>Cell biology international. >Potential of co-culture of nucleus pulposus mesenchymal stem cells and nucleus pulposus cells in hyperosmotic microenvironment for intervertebral disc regeneration
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Potential of co-culture of nucleus pulposus mesenchymal stem cells and nucleus pulposus cells in hyperosmotic microenvironment for intervertebral disc regeneration

机译：高渗微环境中髓核间充质干细胞和髓核细胞共培养对椎间盘再生的潜力

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摘要

Nucleus pulposus mesenchymal stem cells (NPMSCs) are a potential cell source for intervertebral disc (IVD) regeneration, but little is known about their response to IVD-like high osmolarity (400 mOsm). This study was to investigate the viability, proliferation and protein biosynthesis of nucleus pulposus cells (NPCs), NPMSCs and co-cultured NPMSCs-NPCs under IVDlike high osmolarity conditions. NPCs and NPMSCs were isolated and cultured under standard and IVD-like high osmolarity conditions for 1 or 2 weeks. Cell viability was measured by annexin V-FITC and PI staining, and cell proliferation measured by MTTassay. The expression of SOX-9, aggrecan and collagen-II was measured by RT-PCR and Western blot analyses. IVD-like high osmolarity condition slightly inhibited cell viability and decreased the expression of SOX-9, aggrecan and collagen-II at the mRNA and protein levels in all groups compared with standard condition. NPMSCs could tolerate IVD-like high osmolarity, and NPCs-NPMSCs co-culture increased cell proliferation and the expression of SOX-9, aggrecan and collagen-II under both culture conditions, suggesting that co-culture of NPMSCs-NPCs has potential application for IVD regeneration.
机译：髓核间充质干细胞（NPMSC）是椎间盘（IVD）再生的潜在细胞来源，但对其对IVD样高渗透压（400 mOsm）的反应知之甚少。这项研究旨在调查髓核细胞（NPCs），NPMSCs和共培养的NPMSCs-NPC在高渗透压条件下的活力，增殖和蛋白质生物合成。分离NPC和NPMSC，并在标准和类似于IVD的高渗透压条件下培养1或2周。通过膜联蛋白V-FITC和PI染色测量细胞活力，并通过MTTassay测量细胞增殖。通过RT-PCR和蛋白质印迹分析测量SOX-9，聚集蛋白聚糖和胶原II的表达。类似于IVD的高渗透压条件，与标准条件相比，所有组在mRNA和蛋白质水平上均略微抑制细胞活力并降低SOX-9，聚集蛋白聚糖和胶原蛋白II的表达。 NPMSCs可以耐受IVD样的高渗透压，并且NPCs-NPMSCs共同培养在两种培养条件下均能促进细胞增殖以及SOX-9，聚集蛋白聚糖和II型胶原的表达，这表明NPMSCs-NPCs的共同培养具有潜在的应用前景。 IVD再生。

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