Positioning of integrin β1, caveolin-1 and focal adhesion kinase on the adhered membrane of spreading cells

JokhadarS.Z.; MajhencJ.; SvetinaS.; BatistaU.

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Positioning of integrin β1, caveolin-1 and focal adhesion kinase on the adhered membrane of spreading cells

机译：整合素β1，caveolin-1和粘着斑激酶在铺展细胞粘附膜上的定位

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We have investigated the relationship between the spreading of anchorage-dependent cells and the surface-density distribution of plasma membrane adhesion proteins. The surface positioning and density of integrin β1, caveolin-1 (cav-1), the phosphorylated caveolin-1 (p-cav-1) and the focal adhesion kinase (FAK) located on the adhering cell membrane (ACM) of HUVEC cells was studied. Imaging with TIRF microscopy was used, which enabled us to observe a few-nanometers-thin section of the cell above the plasma membrane in combination with image-based analyses. Integrin β1 and cav-1 have spatial interdependenceon the ACM. Cells treatedwith substances that act on cell spreading caused changes inthe size of the ACM area, as well as a redistribution of several proteins under investigation. Changes to the ACM area correlated positively with those to the surface density of the cav-1. The high integrin β1 and the low cav-1 surface density, and vice versa, following the treatments show that the presence of one of them not only spatially excludes, but also reduces, the occurrence of the other protein on the ACM, which indicates a regulative mechanism between integrin β1 and cav-1.

机译：我们已经研究了锚固依赖性细胞的扩散与质膜粘附蛋白的表面密度分布之间的关系。 HUVEC细胞粘附细胞膜（ACM）上整合素β1，caveolin-1（cav-1），磷酸化caveolin-1（p-cav-1）和黏着斑激酶（FAK）的表面定位和密度被研究了。使用TIRF显微镜成像，结合基于图像的分析，使我们能够观察到质膜上方几纳米厚的细胞切片。整联蛋白β1和cav-1与ACM具有空间依赖性。用作用于细胞扩散的物质处理过的细胞引起了ACM区域大小的变化，以及正在研究的几种蛋白质的重新分布。 ACM面积的变化与cav-1的表面密度呈正相关。处理后，高整合素β1和低cav-1表面密度，反之亦然，表明其中之一的存在不仅在空间上排斥，而且减少了ACM上另一种蛋白质的出现，这表明整合素β1和cav-1之间的调控机制。

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《Cell biology international.》 |2013年第12期|共9页
作者
JokhadarS.Z.; MajhencJ.; SvetinaS.; BatistaU.;
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正文语种 eng
中图分类细胞生物学;
关键词
Caveolin-1; Cytoskeleton; Integrin β1; Surface density;

机译：Caveolin-1;细胞骨架;整联蛋白β1;表面密度;

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专利

1. 粘着斑激酶活化对平滑肌细胞粘附和迁移的影响 [J] . 尹航, 汪丽蕙, 霍勇, 中华医学杂志（英文版） . 2002,第004期
2. Positioning of integrin β1, caveolin-1 and focal adhesion kinase on the adhered membrane of spreading cells [J] . JokhadarS.Z., MajhencJ., SvetinaS., Cell biology international. . 2013,第12期

机译：整合素β1，caveolin-1和粘着斑激酶在铺展细胞粘附膜上的定位
3. A New Focal Adhesion Protein That Interacts with Integrin-Linked Kinase and Regulates Cell Adhesion and Spreading [J] . Yizeng Tu, Yao Huang, Yongjun Zhang, Journal of cell biology . 2001,第3期

机译：与整合素链接的激酶相互作用并调节细胞粘附和扩散的新的局灶粘附蛋白。
4. Overexpressed Csk tyrosine kinase is localized in focal adhesions, causes reorganization of alpha v beta 5 integrin, and interferes with HeLa cell spreading. [J] . M Bergman, V Joukov, I Virtanen, Molecular and Cellular Biology . 1995,第2期

机译：过表达的Csk酪氨酸激酶位于粘着斑中，引起αv beta 5整联蛋白的重组，并干扰HeLa细胞扩散。
5. Extracellular matrix proteins mediate osteogenic differentiation of human mesenchymal stem cells on phosphate functionalized gels through integrin mediated focal adhesion kinase signaling [C] . Navakanth Gandavarapu, Kristi S. Anseth Annual meeting of the Society for Biomaterials . 2011

机译：细胞外基质蛋白通过整合素介导的粘着斑激酶信号传导介导人间充质干细胞在磷酸盐功能化凝胶上的成骨分化
6. Using integrins to fine tune CD4+ T cell responses: How the integrin regulatory proteins calpain, PIPKIgamma'90, talin and focal adhesion kinase work together to regulate CD4+ T cell activation. [D] . Wernimont, Sarah A. 2011

机译：使用整合素来微调CD4 + T细胞反应：整合素调节蛋白calpain，PIPKIgamma'90，talin和黏着斑激酶如何协同作用来调节CD4 + T细胞活化。
7. A New Focal Adhesion Protein That Interacts with Integrin-Linked Kinase and Regulates Cell Adhesion and Spreading [O] . Yizeng Tu, Yao Huang, Yongjun Zhang, 2001

机译：与整合素链接的激酶相互作用并调节细胞粘附和扩散的新的局灶性粘附蛋白。
8. The adhering junction dynamics in the testis are regulated by an interplay of beta 1-integrin, focal adhesion kinase (FAK) and the focal adhesion complex (FAC)-associated proteins [O] . Lee W, Cheng C, Siu M, 2002

机译：睾丸中粘附的连接动力学受到β1-整联蛋白，粘着斑激酶（FaK）和粘着斑复合物（FaC）相关蛋白的相互作用的调节。

Positioning of integrin β1, caveolin-1 and focal adhesion kinase on the adhered membrane of spreading cells

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