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首页> 外文期刊>Cell biology international. >Influence of epidermal growth factor (EGF) and hydrocortisone on the co-culture of mature adipocytes and endothelial cells for vascularized adipose tissue engineering
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Influence of epidermal growth factor (EGF) and hydrocortisone on the co-culture of mature adipocytes and endothelial cells for vascularized adipose tissue engineering

机译：表皮生长因子（EGF）和氢化可的松对血管化脂肪组织工程成熟脂肪细胞和内皮细胞共培养的影响

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摘要

The composition of vascularized adipose tissue is still an ongoing challenge as no culture medium is available to supply adipocytes and endothelial cells appropriately. Endothelial cell medium is typically supplemented with epidermal growth factor (EGF) as well as hydrocortisone (HC). The effect of EGF on adipocytes is discussed controversially. Some studies say it inhibits adipocyte differentiation while others reported of improved adipocyte lipogenesis. HC is known to have lipolytic activities, which might result in mature adipocyte dedifferentiation. In this study, we evaluated the influence of EGF and HC on the co-culture of endothelial cells and mature adipocytes regarding their cell morphology and functionality. We showed in mono-culture that high levels of HC promoted dedifferentiation and proliferation of mature adipocytes, whereas EGF seemed to have no negative influence. Endothelial cells kept their typical cobblestone morphology and showed a proliferation rate comparable to the control independent of EGF and HC concentration. In co-culture, HC promoted dedifferentiation of mature adipocytes, which was shown by a higher glycerol release. EGF had no negative impact on adipocyte morphology. No negative impact on endothelial cell morphology and functionality could be seen with reduced EGF and HC supplementation in co-culture with mature adipocytes. Taken together, our results demonstrate that reduced levels of HC are needed for co-culturing mature adipocytes and endothelial cells. In co-culture, EGF had no influence on mature adipocytes. Therefore, for the composition of vascularized adipose tissue constructs, the media with low levels of HC and high or low levels of EGF can be used.
机译：血管化脂肪组织的组成仍然是一个持续的挑战，因为没有可用的培养基来适当地供应脂肪细胞和内皮细胞。内皮细胞培养基通常补充有表皮生长因子（EGF）和氢化可的松（HC）。有争议的讨论了EGF对脂肪细胞的作用。一些研究说它可以抑制脂肪细胞的分化，而另一些则报道可以改善脂肪细胞的脂肪生成。已知HC具有脂解活性，这可能导致成熟的脂肪细胞去分化。在这项研究中，我们评估了EGF和HC对内皮细胞和成熟脂肪细胞共培养的细胞形态和功能的影响。我们在单一培养物中显示，高水平的HC促进成熟脂肪细胞的去分化和增殖，而EGF似乎没有负面影响。内皮细胞保持其典型的鹅卵石形态，并显示出与对照组相当的增殖速率，而与EGF和HC浓度无关。在共培养中，HC促进了成熟脂肪细胞的去分化，甘油的释放量更高。 EGF对脂肪细胞形态没有负面影响。与成熟脂肪细胞共培养时，减少EGF和HC的添加对内皮细胞的形态和功能没有负面影响。两者合计，我们的结果表明，降低胆固醇的水平是共同培养成熟的脂肪细胞和内皮细胞所需要的。在共培养中，EGF对成熟的脂肪细胞没有影响。因此，对于血管化脂肪组织构建体的组成，可以使用HC含量低而EGF含量高或低的培养基。

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