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首页> 外文期刊>Cell and Tissue Research >Development of astroglia heterogeneously expressing Pax2, vimentin and GFAP during the ontogeny of the optic pathway of the lizard (Gallotia galloti): an immunohistochemical and ultrastructural study.
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Development of astroglia heterogeneously expressing Pax2, vimentin and GFAP during the ontogeny of the optic pathway of the lizard (Gallotia galloti): an immunohistochemical and ultrastructural study.

机译：蜥蜴（Gallotia galloti）视神经通路发育过程中异质表达Pax2，波形蛋白和GFAP的星形胶质细胞的发展：一项免疫组织化学和超微结构研究。

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The successful regrowth of retinal ganglion cell (RGC) axons after optic nerve (ON) axotomy in Gallotia galloti indicates a permissive role of the glial environment. We have characterised the astroglial lineage of the lizard optic pathway throughout its ontogeny (embryonic stage 30 [E30] to adults) by using electron microscopy and immunohistochemistry to detect the proliferation marker PCNA (proliferating cell nuclear antigen), the transcription factor Pax2 and the gliofilament proteins vimentin (Vim) and GFAP (glial fibrillary acidic protein). PCNA(+) cells were abundant until E39, with GFAP(+)/PCNA(+) astrocytes being observed between E37 and hatching. Proliferation diminished markedly afterwards, being undetectable in the adult optic pathway. Muller glia of the central retina expressed Pax2 from E37 and their endfeet accumulated Vim from E33 and GFAP from E37 onwards. Astrocytes were absent in the avascular lizard retina, whereas abundant Pax2(+) astrocytes were observed in the ON from E30. A major subpopulation of these astrocytes coexpressed Vim from E35 and also GFAP from E37 onwards; thus the majority of mature astrocytes coexpressed Pax2/Vim/GFAP. The astrocytes were ultrastructurally identified by their gliofilaments, microtubules, dense bodies, desmosomes and glycogen granules, which preferentially accumulated in cell processes. Astrocytes in the adult ON coexpressed both gliofilaments and presented desmosomes indicating a reinforcement of the ON structure; this is physiologically necessary for local adaptation to mechanical forces linked to eye movement. We suggest that astrocytes forming this structural scaffold facilitate the regrowth of RGCs after ON transection.
机译：gallotia galloti视神经（ON）轴切术后视网膜神经节细胞（RGC）轴突的成功再生表明神经胶质环境的允许作用。我们已经通过使用电子显微镜和免疫组织化学检测增殖标志物PCNA（增殖细胞核抗原），转录因子Pax2和神经胶质丝形成了蜥蜴视神经通路的星形胶质细胞谱系，贯穿其个体发育（胚胎期30 [E30]成人）蛋白波形蛋白（Vim）和GFAP（神经胶质纤维酸性蛋白）。直到E39为止，PCNA（+）细胞数量丰富，在E37和孵化之间观察到了GFAP（+）/ PCNA（+）星形胶质细胞。之后，增殖显着减少，在成人视神经通路中无法检测到。中央视网膜的穆勒神经胶质从E37表达Pax2，从E33到它们的末端积累Vim，从E37开始积累GFAP。在无血管的蜥蜴视网膜中没有星形胶质细胞，而从E30开始在ON中观察到大量的Pax2（+）星形胶质细胞。这些星形胶质细胞的主要亚群从E35共表达Vim，从E37开始共表达GFAP。因此，大多数成熟的星形胶质细胞共表达Pax2 / Vim / GFAP。通过星形胶质细胞，微管，致密体，桥粒和糖原颗粒对星形胶质细胞进行超微结构鉴定，它们优先积累在细胞过程中。成年ON中的星形胶质细胞共表达神经丝和呈桥粒状，表明ON结构增强。对于局部适应与眼睛运动有关的机械力，这在生理上是必需的。我们建议形成这种结构支架的星形胶质细胞促进ON横切后RGC的再生。

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