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首页> 外文期刊>Cell and Tissue Research >Expression and localization of transcription factors SNAIL and SLUG in mouse ovaries and pre-implantation embryos.
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Expression and localization of transcription factors SNAIL and SLUG in mouse ovaries and pre-implantation embryos.

机译：转录因子SNAIL和SLUG在小鼠卵巢和植入前胚胎中的表达和定位。

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摘要

SNAIL and SLUG are zinc-finger transcription factors that participate in the regulation of cell division, cell survival, mesoderm formation and epithelial-to-mesenchymal transition. We investigate the expression of SNAIL and SLUG during follicular maturation, ovulation and luteinization in the ovaries of both neonatal mice and gonadotropin-induced immature mice. Furthermore, we examine the expression and localization of these transcription factors during early embryonic cleavage. Our data demonstrate that both SNAIL and SLUG are present in the epithelial cells of the ovarian surface in immature mice. SNAIL is first evident in the interstitial cells and theca cells by postnatal day (PD) 6 and then appears in the oocytes by PD 8, remaining at a constant expression level for all stages studied thereafter. SLUG is expressed in oocytes as early as PD 1. Its expression also increases with the development of the follicles in theca and interstitial cells but not in granulosa cells. In gonadotropin-induced immature mice, both SNAIL and SLUG are expressed in the corpora lutea. During early embryo cleavage, SNAIL occurs in the nucleus and cytoplasm of the majority of the embryo, excluding the nucleolus from the germinal vesicle breakdown (GVBD) to the 8-cell stage and is then localized in the cytoplasm during the morula stage and in the nucleus during the blastocyst stage. SLUG has an identical expression pattern as SNAIL from GVBD until the morula stage, except that it is localized in the cytoplasm during the blastocyst stage. Taken together, these different localization patterns suggest that SNAIL and SLUG probably play important roles during follicular development, luteinization and early embryonic development.
机译：SNAIL和SLUG是锌指转录因子，参与细胞分裂，细胞存活，中胚层形成和上皮-间充质转化的调控。我们调查了新生小鼠和促性腺激素诱导的未成熟小鼠卵巢中卵泡成熟，排卵和黄体生成过程中SNAIL和SLUG的表达。此外，我们检查早期胚胎卵裂期间这些转录因子的表达和定位。我们的数据表明，SNAIL和SLUG都存在于未成熟小鼠卵巢表面的上皮细胞中。 SNAIL首先在出生后第6天出现在间质细胞和卵泡膜细胞中，然后在PD 8出现在卵母细胞中，此后研究的所有阶段均保持恒定的表达水平。 SLUG最早在PD 1时就在卵母细胞中表达。其表达也随着卵泡和间质细胞中卵泡的发育而增加，而在颗粒细胞中则没有。在促性腺激素诱导的未成熟小鼠中，SNAIL和SLUG均在黄体中表达。在早期卵裂过程中，SNAIL发生在大多数胚的核和细胞质中，不包括从生小泡破裂（GVBD）到8细胞期的核仁，然后在桑ula期和细胞质中定位于细胞质中。胚泡阶段的细胞核。直到桑期，SLUG的表达模式与GVBD的SNAIL相同，不同的是它在胚泡期位于细胞质中。综上所述，这些不同的定位模式表明SNAIL和SLUG可能在卵泡发育，黄素化和早期胚胎发育过程中发挥重要作用。

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