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首页> 外文期刊>Cell and Tissue Research >Morphological and molecular investigations of the holocephalan elephant fish nephron: the existence of a countercurrent-like configuration and two separate diluting segments in the distal tubule
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Morphological and molecular investigations of the holocephalan elephant fish nephron: the existence of a countercurrent-like configuration and two separate diluting segments in the distal tubule

机译：大头象鱼肾的形态和分子研究：远端小管中存在逆流样构型和两个单独的稀释段

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In marine cartilaginous fish, reabsorption of filtered urea by the kidney is essential for retaining a large amount of urea in their body. However, the mechanism for urea reabsorption is poorly understood due to the complexity of the kidney. To address this problem, we focused on elephant fish (Callorhinchus milii) for which a genome database is available, and conducted molecular mapping of membrane transporters along the different segments of the nephron. Basically, the nephron architecture of elephant fish was similar to that described for elasmobranch nephrons, but some unique features were observed. The late distal tubule (LDT), which corresponded to the fourth loop of the nephron, ran straight near the renal corpuscle, while it was convoluted around the tip of the loop. The ascending and descending limbs of the straight portion were closely apposed to each other and were arranged in a countercurrent fashion. The convoluted portion of LDT was tightly packed and enveloped by the larger convolution of the second loop that originated from the same renal corpuscle. In situ hybridization analysis demonstrated that co-localization of Na+,K+,2Cl(-) cotransporter 2 and Na+/K+-ATPase alpha 1 subunit was observed in the early distal tubule and the posterior part of LDT, indicating the existence of two separate diluting segments. The diluting segments most likely facilitate NaCl absorption and thereby water reabsorption to elevate urea concentration in the filtrate, and subsequently contribute to efficient urea reabsorption in the final segment of the nephron, the collecting tubule, where urea transporter-1 was intensely localized.
机译：在海洋软骨鱼类中，肾脏对过滤后的尿素的重吸收对于在体内保留大量尿素至关重要。然而，由于肾脏的复杂性，对尿素重吸收的机制了解甚少。为了解决这个问题，我们将重点放在大象鱼（Callorhinchus milii）上，该大象的基因组数据库可用，并对沿肾单位不同部分的膜转运蛋白进行了分子定位。基本上，象鱼的肾单位结构与描述的弹bra肾单位相似，但观察到一些独特的特征。远端肾小管（LDT）对应于肾单位的第四环，在肾小体附近笔直地延伸，而在环的尖端周围盘旋。笔直部分的上升和下降分支彼此紧密并置，并以逆流方式排列。 LDT的旋绕部分被来自同一肾小球的第二环的较大旋绕紧密地包裹和包裹。原位杂交分析表明，在远端远端小管和LDT后部观察到Na +，K +，2Cl（-）共转运蛋白2和Na + / K + -ATPaseα1亚基的共定位，表明存在两个单独的稀释液段。稀释段最有可能促进NaCl的吸收，从而促进水的重吸收，从而提高滤液中的尿素浓度，并随后在肾单位的最后一个部分，即尿素转运蛋白1强烈定位的收集管中，促进有效的尿素重吸收。

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