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首页> 外文期刊>Cell and Tissue Research >Combined effect of ligament stem cells and umbilical-cord-blood-derived CD34+cells on ligament healing
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Combined effect of ligament stem cells and umbilical-cord-blood-derived CD34+cells on ligament healing

机译：韧带干细胞和脐带血CD34 +细胞对韧带愈合的联合作用

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Transplantation of ligament-tissue-derived stem cells has become a promising approach in the repair of injured ligament. Neovascularization plays an important role in ligament healing and remodeling. Recently, human umbilical-cord-blood-derived CD34+ cells have been reported to contribute to neoangiogenesis. Therefore, we performed a series of experiments to test our hypothesis that the combination of medial collateral ligament stem cells (MCL-SCs) and umbilical-cord-blood-derived CD34+ cells has synergistic effects on tendon healing. MCL-SCs and umbilical-cord-blood-derived CD34+ cells were isolated and cultured. Rat MCL injury was treated by MCL-SCs and/or CD34+ cells. Response to the cell therapy was assessed by gross observation, histological evaluation and biomechanical testing at 2 and 4 weeks after each treatment. Although each cell therapy group induced macroscopic and morphological recovery in healing MCLs, the combined use of MCL-SCs/CD34+ cells led to further improvement in healing quality. Capillary density was significantly higher in the CD34+ cell transplantation groups than in the other groups at week 2. Biomechanical testing demonstrated that the failure load of the healing ligament was greatest in the combination therapy group. The combination of MCL-SCs and CD34+ cells as a cell therapeutic thus enhances healing and restores biomechanical function toward normal after MCL injury. The findings obtained in our study suggest that the combination of MCL-SCs and CD34+ cells transplantation represents a promising strategy for ligament injury.
机译：韧带组织干细胞的移植已成为修复受损韧带的一种有前途的方法。新血管形成在韧带愈合和重塑中起重要作用。近来，已报道人脐带血来源的CD34 +细胞有助于新血管生成。因此，我们进行了一系列实验来检验我们的假设，即内侧副韧带干细胞（MCL-SC）与脐带血来源的CD34 +细胞的组合对肌腱愈合具有协同作用。分离并培养了MCL-SC和脐带血来源的CD34 +细胞。用MCL-SC和/或CD34 +细胞治疗大鼠MCL损伤。每次治疗后2和4周，通过肉眼观察，组织学评估和生物力学测试评估对细胞疗法的反应。尽管每个细胞治疗组都能在愈合的MCL中引起宏观和形态上的恢复，但MCL-SCs / CD34 +细胞的联合使用可进一步改善愈合质量。在第2周时，CD34 +细胞移植组的毛细血管密度明显高于其他组。生物力学测试表明，联合治疗组中愈合韧带的衰竭负荷最大。因此，MCL-SCs和CD34 +细胞的组合作为细胞治疗剂可增强愈合，并在MCL损伤后恢复正常的生物力学功能。我们的研究结果表明，MCL-SCs和CD34 +细胞移植的结合代表了韧带损伤的有前途的策略。

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