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首页> 外文期刊>Cell and Tissue Research >Expression and localization of matrix metalloproteinases (MMP-2,-7,-9) and their tissue inhibitors (TIMP-2,-3) in the chicken oviduct during maturation
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Expression and localization of matrix metalloproteinases (MMP-2,-7,-9) and their tissue inhibitors (TIMP-2,-3) in the chicken oviduct during maturation

机译：鸡输卵管成熟过程中基质金属蛋白酶（MMP-2，-7，-9）及其组织抑制剂（TIMP-2，-3）的表达和定位

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摘要

Although participation of matrix metalloproteinases (MMPs) in reproductive tract remodeling has been strongly suggested in mammalian species, the role of MMPs in the avian oviduct has received little attention. To gain a better understanding of the potential role of the MMP system in avian oviduct development, mRNA and protein expression, localization of selected MMPs and their tissue inhibitors (TIMPs), and gelatinolytic activity in the oviduct of growing chickens were examined. The oviducts were collected from Hy-Line Brown hens before (10, 12, 14 and 16 weeks of age) and after (week 17) the onset of egg laying. The MMP-2, -7, -9 and TIMP-2 and -3 genes were found to be differentially expressed in all examined oviductal sections: the infundibulum, magnum, isthmus and shell gland on both mRNA (by real time polymerase chain reaction) and protein (by western blotting and immunohistochemistry) levels. In the course of oviduct development, the relative expression of all genes decreased in most sections. Protein level of MMP-9 was diminished, while MMP-7 and TIMP-3 were elevated in the oviduct of growing birds. MMP-2 and TIMP-2 protein levels remained constant, with a slight increase in MMP-2 concentration just before reaching maturity. The relative activity of MMP-2 and -9 (assessed by gelatin zymography) was higher (P < 0.05, P < 0.01) in immature birds compared with adults. Immunohistochemistry demonstrated cell- and tissue-specific localization of MMPs and TIMPs in the wall of the chicken oviduct. We concluded that changes in the expression of examined MMPs and their inhibitors, as well as alterations in MMP activity occurring simultaneously with changes in the morphology of the chicken oviduct, suggest the involvement of the MMP system in the proper development and functioning of this organ. Mechanisms regulating the expression and activity of MMPs require further clarification.
机译：尽管在哺乳动物物种中强烈建议基质金属蛋白酶（MMPs）参与生殖道重塑，但MMPs在禽输卵管中的作用很少受到关注。为了更好地了解MMP系统在禽输卵管发育中的潜在作用，mRNA和蛋白质表达，所选MMP及其组织抑制剂（TIMPs）的定位以及成年鸡输卵管中的明胶分解活性。在产卵开始之前（第10、12、14和16周龄）和之后（第17周）从Hy-Line Brown母鸡收集输卵管。 MMP-2，-7，-9和TIMP-2和-3基因在所有检查的输卵管切片中均表达差异：两个mRNA上的漏斗，大瓶盖，峡部和壳腺（通过实时聚合酶链反应）和蛋白质（通过蛋白质印迹和免疫组化）水平。在输卵管发育过程中，大多数部分中所有基因的相对表达均下降。生长中鸟类的输卵管中MMP-9的蛋白质水平降低，而MMP-7和TIMP-3升高。 MMP-2和TIMP-2蛋白水平保持恒定，MMP-2浓度在即将达到成熟之前略有增加。与成年相比，未成熟禽类中MMP-2和-9的相对活性（通过明胶酶谱评估）更高（P <0.05，P <0.01）。免疫组织化学表明，MMPs和TIMPs在鸡输卵管壁中具有细胞和组织特异性定位。我们得出的结论是，受检MMP及其抑制剂表达的变化以及MMP活性的改变与鸡输卵管形态的变化同时发生，表明MMP系统参与了该器官的正常发育和功能。调节MMPs表达和活性的机制需要进一步阐明。

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