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首页> 外文期刊>Cell biology international. >Effects of ascorbic acid and ascorbic acid 2-phosphate, a long-acting vitamin C derivative, on the proliferation and differentiation of human osteoblast-like cells.
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Effects of ascorbic acid and ascorbic acid 2-phosphate, a long-acting vitamin C derivative, on the proliferation and differentiation of human osteoblast-like cells.

机译：长效维生素C衍生物抗坏血酸和2-磷酸抗坏血酸对人成骨样细胞增殖和分化的影响。

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摘要

In order to investigate the effect of ascorbic acid (AsA) and ascorbic acid 2-phosphate (Asc 2-P), a long-acting vitamin C derivative, on the growth and differentiation of human osteoblast-like cells, we supplemented the culture medium of MG-63 cells with various concentrations (0.25 to 1 mM) of these factors. Asc 2-P significantly stimulated nascent cell growth at all concentrations in the presence of fetal bovine serum (FBS). On the other hand, AsA showed a growth repressive effect depending on its concentration, and that of FBS. Asc 2-P also increased expression of osteoblast differentiation markers, such as collagen synthesis and alkaline phosphatase (ALP) activity. These stimulative activities of Asc 2-P were attenuated by inhibitors of collagen synthesis, indicating that these effects were dependent on collagen synthesis. Electron micrographs of the cells showed the formation of a three-dimensional tissue-like structure endowed with a mature extracellular matrix in the presence of Asc 2-P.
机译：为了研究长效维生素C衍生物抗坏血酸（AsA）和抗坏血酸2-磷酸（Asc 2-P）对人成骨样细胞生长和分化的影响，我们添加了培养基这些因子具有各种浓度（0.25至1 mM）的MG-63细胞。在胎牛血清（FBS）存在下，Asc 2-P在所有浓度下均能显着刺激新生细胞的生长。另一方面，AsA根据其浓度和FBS的浓度显示出抑制生长的作用。 Asc 2-P还增加了成骨细胞分化标志物的表达，例如胶原蛋白合成和碱性磷酸酶（ALP）活性。 Asc 2-P的这些刺激活性被胶原蛋白合成抑制剂减弱，表明这些作用取决于胶原蛋白合成。细胞的电子显微照片显示，在Asc 2-P存在下，形成了具有成熟细胞外基质的三维组织样结构。

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