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首页> 外文期刊>Cell biology international. >Dual apoptotic effect of Xrel3 c-Rel/NF-kappaB homolog in human cervical cancer cells.
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Dual apoptotic effect of Xrel3 c-Rel/NF-kappaB homolog in human cervical cancer cells.

机译：Xrel3 c-Rel / NF-kappaB同源物在人宫颈癌细胞中的双重凋亡作用。

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Cervical cancer is one of the most common cancers affecting a woman's reproductive organs. Despite its frequency and recurrence, the death rate has been declining over the past 40 years, due to early detection and treatment. In a previous report [Shehata Marlene, Shehata Marian, Shehata Fady, Pater Alan. Apoptosis effects of Xrel3 c-Rel/Nuclear factor-kappa B homolog in human cervical cancer cells. Cell Biology International, in press], we studied the role of the NF-kappaB gene family in HeLa human cervical cancer cells, using the Xrel3 c-Rel homologue of Xenopus laevis. These results showed that the expression of Xrel3/c-Rel slowed cell growth, consistent with an upregulated expression of the cell cycle inhibitor p21 and the activated poly(ADP-ribose) polymerase (PARP) apoptosis effector. However, in this report, we examined more apoptotic and anti-apoptotic factors acting upstream and downstream in apoptosis pathways after cisplatin treatment of HeLa cervical cancer cells. After 1 microM cisplatin treatment, Xrel3 had an anti-apoptotic effect, based on significantly lower levels of apoptotic proteins, including caspase-8, caspase-3 and p21. Anti-apoptotic BAG-1 isoforms were upregulated. After 5 microM cisplatin treatment, expression of HeLa Xrel3 had an apoptotic effect, based on significantly increased expression of the cell cycle inhibitor p21 and apoptotic proteins, including cleaved PARP, caspase-8, and caspase-3. However, anti-apoptotic Bcl-2 and Bcl-X(L) were elevated and the cell cycle regulator cyclin D1 was slightly upregulated with both 1 and 5 microM cisplatin treatment. The HPV E6 oncoprotein showed no significant changes. These results support previous conclusions on the potential anti-apoptotic effects of c-Rel/NF-kappaB in mild stress environments, as opposed to the apoptotic effects associated with high stress conditions [Lake BB, Ford R, Kao KR. Xrel3 is required for head development in Xenopus laevis. Development 2001; 128(2), 263-73.]. Thus, c-Rel/NF-kappaB may potentially be of clinical significance in chemotherapy.
机译：宫颈癌是影响女性生殖器官的最常见癌症之一。尽管其发病率和复发率很高，但由于早期发现和治疗，死亡率在过去40年中一直在下降。在以前的报告中[Shehata Marlene，Shehata Marian，Shehata Fady和Pater Alan。 Xrel3 c-Rel /核因子-κB同源物在人宫颈癌细胞中的凋亡作用。国际细胞生物学，正在出版中]，我们使用非洲爪蟾的Xrel3 c-Rel同源物研究了NF-kappaB基因家族在HeLa人宫颈癌细胞中的作用。这些结果表明，Xrel3 / c-Rel的表达减慢了细胞的生长，这与细胞周期抑制剂p21和活化的聚（ADP-核糖）聚合酶（PARP）凋亡效应子的表达上调一致。但是，在本报告中，我们研究了顺铂治疗HeLa宫颈癌细胞后在凋亡途径中上游和下游起作用的更多凋亡和抗凋亡因子。经过1 microM顺铂处理后，Xrel3具有抗凋亡作用，其基础是凋亡蛋白的水平明显降低，包括caspase-8，caspase-3和p21。抗凋亡BAG-1亚型上调。经过5 microM顺铂处理后，HeLa Xrel3的表达具有凋亡作用，这是基于细胞周期抑制剂p21和凋亡蛋白（包括裂解的PARP，caspase-8和caspase-3）的表达显着增加。但是，抗凋亡的Bcl-2和Bcl-X（L）升高，并且细胞周期调节剂cyclin D1在1和5 microM顺铂处理下均略有上调。 HPV E6癌蛋白无明显变化。这些结果支持了先前有关c-Rel / NF-kappaB在轻度应激环境下的潜在抗凋亡作用的结论，而不是与高应激条件相关的凋亡作用[湖BB，福特R，花王KR]。 Xrel3是非洲爪蟾头部开发所必需的。发展2001; 128（2），263-73。]。因此，c-Rel /NF-κB在化学疗法中可能具有临床意义。

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