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首页> 外文期刊>Cell biology international. >Epigallocatechin gallate-induced apoptosis does not affect adipocyte conversion of preadipocytes
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Epigallocatechin gallate-induced apoptosis does not affect adipocyte conversion of preadipocytes

机译：表没食子儿茶素没食子酸酯诱导的细胞凋亡不影响前脂肪细胞的脂肪细胞转化

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摘要

In the present study, we examined the effect of epigallocatechin gallate (EGCG) on the growth and differentiation of human preadipocyte cells, AML-I. EGCG exhibited cytotoxic activity on AML-I cells, accompanied by the appearance of characteristics of apoptosis by Annexin V–FITC staining method. Among apoptosis-related proteins examined, loss of NF-κB and p-Akt, and accumulation of Bad were displayed in EGCG-treated cells by Western blot analysis. Among 6 structure-related catechins including catechin (C), epicatechin (EC), catechin gallate (CG), epigallocatechin (EGC), epicatechin gallate (ECG) and EGCG, the catechins containing galloyl moiety exhibited apoptotic capacity. Interestingly, exposure of AML-I to EGCG increased the amounts of cytoplasmic lipid droplets as well as the expression of fatty acid synthase and peroxisome proliferator activated receptor-γ proteins. Our results suggest that EGCG induces growth arrest and apoptosis, but does not affect adipocyte conversion of preadipocytes.
机译：在本研究中，我们检查了表没食子儿茶素没食子酸酯（EGCG）对人前脂肪细胞细胞AML-1的生长和分化的影响。 EGCG对AML-1细胞表现出细胞毒活性，并通过Annexin V-FITC染色方法出现凋亡特征。通过Western blot分析，在检测的凋亡相关蛋白中，EGCG处理的细胞中显示出NF-κB和p-Akt的丢失以及Bad的积累。在6种与结构相关的儿茶素中，包括儿茶素（C），表儿茶素（EC），没食子儿茶素（CG），表没食子儿茶素（EGC），表儿茶素没食子酸酯（ECG）和EGCG，其中含有没食子酰基部分的儿茶素具有凋亡能力。有趣的是，将AML-1暴露于EGCG会增加细胞质脂质滴的数量以及脂肪酸合酶和过氧化物酶体增殖物激活的受体γ蛋白的表达。我们的结果表明，EGCG诱导生长停滞和凋亡，但不影响前脂肪细胞的脂肪细胞转化。

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