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首页> 外文期刊>Cell biology international. >Serum-free medium with osteogenic supplements induces adipogenesis in rat bone marrow stromal cells.
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Serum-free medium with osteogenic supplements induces adipogenesis in rat bone marrow stromal cells.

机译：含成骨补剂的无血清培养基可诱导大鼠骨髓基质细胞中的脂肪形成。

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摘要

Adult BMSCs (bone marrow stromal cells) contain MSCs (mesenchymal stem cells). MSCs can differentiate into osteoblasts, chondrocytes, adipocytes and myoblasts and are thus considered useful in tissue engineering for therapeutic and clinical purposes. FCS (fetal calf serum) is usually included in the differentiation medium, but the clinical application of FCS may pose a problem for some patients. To improve the efficiency and safety of BMSC cultivation, the effect of SF (serum-free) conditions on the osteogenic differentiation of rat BMSCs was examined. In the presence of 10% FCS and osteogenic supplements, the cells formed mineralized von Kossa-positive deposits. Under SF conditions with osteogenic supplementation, however, the cells possessed cytosolic lipid vacuoles that could be stained with Oil Red O. The mRNA expression of PPARgamma (peroxisome proliferator-activated receptor gamma), an adipogenic marker, increased under the SF condition with osteogenic supplements. These data indicate that rat BMSCs differentiate into adipocytes under SF conditions even with osteogenic supplementation and that FCS is needed to induce proper osteogenic differentiation in rat BMSCs.
机译：成年BMSC（骨髓基质细胞）含有MSC（间充质干细胞）。 MSC可以分化为成骨细胞，软骨细胞，脂肪细胞和成肌细胞，因此被认为可用于治疗和临床目的的组织工程。 FCS（胎牛血清）通常包含在分化培养基中，但是FCS的临床应用可能会给某些患者带来问题。为了提高BMSC培养的效率和安全性，研究了SF（无血清）条件对大鼠BMSCs成骨分化的影响。在10％FCS和成骨补充剂存在下，细胞形成矿化的von Kossa阳性矿床。然而，在有成骨性补充剂的SF条件下，细胞具有可被油红O染色的胞浆脂质液泡。成骨性补充剂在SF条件下增加了成脂标记物PPARgamma（过氧化物酶体增殖物激活的受体γ）的mRNA表达。 。这些数据表明，即使在有成骨作用的补充下，大鼠BMSC在SF条件下也可分化为脂肪细胞，并且需要FCS诱导大鼠BMSC进行适当的成骨分化。

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