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首页> 外文期刊>Cell biology international. >Nuclear structures in Tribolium castaneum oocytes.
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Nuclear structures in Tribolium castaneum oocytes.

机译：Tribolium castaneum卵母细胞的核结构。

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摘要

The first ultrastructural and immunomorphological characteristics of the karyosphere (karyosome) and extrachromosomal nuclear bodies in the red flour beetle, Tribolium castaneum, are presented. The karyosphere forms early in the diplotene stage of meiotic prophase by the gathering of all oocyte chromosomes in a limited nuclear volume. Using the BrUTP assay, T. castaneum oocyte chromosomes united in the karyosphere maintain their transcriptional activity until the end of oocyte growth. Hyperphosphorylated RNA polymerase II and basal transcription factors (TFIID and TFIIH) were detected in the perichromatin region of the karyosphere. The T. castaneum karyosphere has an extrachromosomal capsule that separates chromosomes from the rest of the nucleoplasm. Certain structural proteins (F-actin, lamin B) were found in the capsule. Unexpectedly, the karyosphere capsule in T. castaneum oocytes was found to be enriched in TMG-capped snRNAs, which suggests that the capsule is not only a structural support for the karyosphere, but may be involved in biogenesis of snRNPs. We also identified the counterparts of 'universal' extrachromosomal nuclear domains, Cajal bodies (CBs) and interchromatin granule clusters (IGCs). Nuclear bodies containing IGC marker protein SC35 display some features unusual for typical IGCs. SC35 domains in T. castaneum oocytes are predominantly fibrillar complex bodies that do not contain trimethyl guanosine (TMG)-capped small nuclear (sn) RNAs. Microinjections of 2'-O-methyl (U)22 probes into the oocytes allowed revealing poly(A)(+) RNAs in these nuclear domains. Several proteins related to mRNA export (heterogeneous ribonucleoprotein core protein A1, export adapters Y14 and Aly and export receptor NXF1) were also detected there. We believe that unusual SC35 nuclear domains of T. castaneum oocytes are possibly involved in mRNP but not snRNP biogenesis.
机译：介绍了红色面粉甲虫Tribolium castaneum的核圈（染色体）和染色体外核体的第一个超微结构和免疫形态学特征。核球层在减数分裂前期的二倍体阶段早期形成，其方式是在有限的核体积中聚集所有卵母细胞染色体。使用BrUTP分析，在核球体中团结的锥栗锥虫卵母细胞染色体保持其转录活性，直至卵母细胞生长结束。在核层的染色质周围区域检测到了超磷酸化的RNA聚合酶II和基础转录因子（TFIID和TFIIH）。 T. castaneum核仁层有一个染色体外的囊，它将染色体与其余核质分开。在胶囊中发现了某些结构蛋白（F-肌动蛋白，层粘连蛋白B）。出乎意料的是，发现cast树卵母细胞的核球囊富含TMG封端的snRNA，这表明该囊不仅是核球的结构支持，而且可能参与了snRNPs的生物发生。我们还确定了“通用”染色体外核域，Cajal体（CBs）和染色质间颗粒簇（IGC）的对应物。含有IGC标记蛋白SC35的核体显示出一些典型IGC所不具备的特征。栗树卵母细胞中的SC35结构域主要是原纤维复合体，不含三甲基鸟苷（TMG）封端的小核（sn）RNA。将2'-O-甲基（U）22探针显微注射到卵母细胞中可以揭示这些核域中的poly（A）（+）RNA。在那里还检测到几种与mRNA输出有关的蛋白（异质核糖核蛋白核心蛋白A1，输出接头Y14和Aly和输出受体NXF1）。我们相信，T。castaneum卵母细胞异常的SC35核域可能参与mRNP，但不参与snRNP生物发生。

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