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首页> 外文期刊>Cell biology international. >Identification and analysis of glycogen synthase kinase 3 beta1 interactome
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Identification and analysis of glycogen synthase kinase 3 beta1 interactome

机译：糖原合酶激酶3 beta1相互作用组的鉴定与分析

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摘要

Glycogen synthase kinase-3 beta (GSK3b) was initially identified as a key protein in glucose metabolism. GSK3b might be involved in cell growth, motility and apoptosis. Systematic identification of GSK3b-associated proteins is crucial for the exhaustive understanding of its functions. Using GST pull-down experiment and LCMS/MS analysis coupled to bioinformatics tools, we have identified 114 proteins that interacted with GSK3b1 in hepatocellular carcinoma HepG2 cells. Most of the identified proteins are implicated in metabolic process, whereas other proteins are important for cell proliferation or migration, and have been associated with cancer development and metastasis. Several representative proteins, such as hnRNPK, PCNA, Ezrin and STAT1, have been confirmed to interact with GSK-3b1 by co-immunoprecipitation in HepG2 cells. Further studies of these interactions may discover the precise roles and the underlying mechanisms of GSK-3b1 in tumour growth and metastasis.
机译：糖原合酶激酶3 beta（GSK3b）最初被确定为葡萄糖代谢中的关键蛋白。 GSK3b可能参与细胞生长，运动和凋亡。系统鉴定GSK3b相关蛋白对于彻底了解其功能至关重要。使用GST下拉实验和结合生物信息学工具的LCMS / MS分析，我们确定了114种与GSK3b1在肝细胞癌HepG2细胞中相互作用的蛋白。大多数鉴定出的蛋白质与代谢过程有关，而其他蛋白质对于细胞增殖或迁移很重要，并且与癌症的发展和转移有关。通过在HepG2细胞中进行免疫共沉淀，已经确认了几种代表性的蛋白质，例如hnRNPK，PCNA，Ezrin和STAT1与GSK-3b1相互作用。对这些相互作用的进一步研究可能会发现GSK-3b1在肿瘤生长和转移中的确切作用及其潜在机制。

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