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首页> 外文期刊>Cell biology international. >Pulmonary surfactant expression analysis-Role of cell-cell interactions and 3-D tissue-like architecture
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Pulmonary surfactant expression analysis-Role of cell-cell interactions and 3-D tissue-like architecture

机译：肺表面活性剂表达分析-细胞-细胞相互作用和3-D组织样结构的作用

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摘要

Surfactant production is important in maintaining alveolar function both in vivo and in vitro, but surfactant expression is the primary property lost by alveolar Type II Pneumocytes in culture and its maintenance is a functional requirement. To develop a functional tissue-like model, the in vivo cell-cell interactions and three dimensional architecture has to be reproduced. To this end, 3D button-shaped synthetic gelatin vinyl acetate (GeVAc) co-polymer scaffold was seeded with different types of lung cells. Functionality of the construct was studied under both static and dynamic conditions. The construct was characterized by Environmental Scanning Electron and fluorescent microscopy, and functionality of the system was analyzed by studying mRNA modulations of all four surfactant genes A, B, C, and D by real time-PCR and varying culture conditions. The scaffold supports alveolar cell adhesion and maintenance of cuboidal morphology, and the alveolar-specific property of surfactant synthesis, which would otherwise be rapidly lost in culture. This is a novel 3D system that expresses all 4 surfactants for a culture duration of 3 weeks.
机译：表面活性剂的产生对于维持体内和体外的肺泡功能均很重要，但是表面活性剂的表达是培养中的肺泡II型肺细胞丧失的主要特性，其维持是一项功能需求。为了建立功能性的组织样模型，必须复制体内细胞间相互作用和三维结构。为此，将3D纽扣形合成明胶醋酸乙烯酯（GeVAc）共聚物支架植入了不同类型的肺细胞。在静态和动态条件下研究了构建体的功能。通过环境扫描电子显微镜和荧光显微镜对构建体进行表征，并通过实时PCR和变化的培养条件研究所有四个表面活性剂基因A，B，C和D的mRNA调节，从而分析了系统的功能。支架支持肺泡细胞粘附和维持立方体形状，以及表面活性剂合成的肺泡特异性特性，否则该特性会在培养中迅速丧失。这是一个新颖的3D系统，可在3周的培养时间内表达所有4种表面活性剂。

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