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首页> 外文期刊>Cell biology international. >Melanocyte migration is influenced by E-cadherin-dependent adhesion of keratinocytes in both two- and three-dimensional in vitro wound models
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Melanocyte migration is influenced by E-cadherin-dependent adhesion of keratinocytes in both two- and three-dimensional in vitro wound models

机译：在二维和三维体外伤口模型中，黑色素细胞迁移受角化细胞依赖E-​​钙粘着蛋白的粘附影响

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During wound healing, melanocytes are required to migrate into the wounded area that is still in the process of re-construction. The role and behaviour of melanocytes during this process is poorly understood, that is, whether melanocyte migration into the wound is keratinocyte-dependent or not. This paper attempts, through the use of both two- and three-dimensional in vitro models, to understand the role and behaviour of melanocytes during the process of wound healing. In addition, it sheds light on whether keratinocytes influence/contribute toward melanocyte migration and ultimately wound healing. Scratch assays were performed to analyse migration and Western blot analyses measured cellular E-cadherin expression. Immunohistochemistry was used to analyse the in vivo 3D wound healing effect. Scratch assays performed on co-cultures of melanocytes and keratinocytes demonstrated that melanocytes actively migrated, with the use of their dendrites, into the scratch ahead of the proliferating keratinocyte sheet. Migration of the melanocyte into the wound bed was accompanied by loss of attachment to keratinocytes at the wound front with concomitant downregulation of E-cadherin expression as observed through immunocytochemistry. This result suggests that, in vitro, melanocyte migration occurs independently of keratinocytes but that the migration is influenced by keratinocyte E-cadherin expression. We now demonstrate that melanocyte migration during re-pigmentation is an active process, and suggest that targeting of mechanisms involved in active melanocyte migration (e.g. the melanocyte dendrite) may enhance the re-pigmentation process.
机译：在伤口愈合期间，需要黑素细胞迁移到仍在重建过程中的伤口区域。黑色素细胞在此过程中的作用和行为知之甚少，也就是说，黑色素细胞迁移到伤口中是否与角质形成细胞有关。本文试图通过使用二维和三维体外模型来了解黑色素细胞在伤口愈合过程中的作用和行为。此外，它揭示了角质形成细胞是否影响/有助于黑色素细胞迁移并最终促进伤口愈合。进行刮擦分析以分析迁移，并且Western印迹分析测量了细胞E-钙粘蛋白的表达。免疫组织化学用于分析体内3D伤口愈合效果。对黑素细胞和角质形成细胞共培养进行的划痕分析表明，黑素细胞利用其树突主动迁移到角质形成细胞表层之前的划痕中。通过免疫细胞化学观察，黑素细胞向伤口床的迁移伴随着与伤口前角质形成细胞的附着丧失，并伴随着E-钙黏着蛋白表达的下调。该结果表明，在体外，黑素细胞迁移独立于角质形成细胞而发生，但是该迁移受角质形成细胞E-钙粘着蛋白表达的影响。现在我们证明在色素沉着过程中黑素细胞的迁移是一个活跃的过程，并建议针对参与活跃的色素黑素迁移的机制（例如黑色素细胞的树突）的靶向可能会增强色素沉着的过程。

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