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首页> 外文期刊>Cell biology international. >Fluvastatin delays propagation of viral infection in isolated rat FDB myofibers but does not affect exocytic membrane trafficking
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Fluvastatin delays propagation of viral infection in isolated rat FDB myofibers but does not affect exocytic membrane trafficking

机译：氟伐他汀延缓了分离的大鼠FDB肌纤维中病毒感染的传播，但不影响胞外膜运输

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We have utilized the enveloped viral model to study the effect of fluvastatin on membrane trafficking in isolated rat myofibers. Our immunofluorescence studies constantly showed that infections in myofibers, which were treated with fluvastatin prior and during the infection with either vesicular stomatitis virus (VSV) or influenza A virus, propagated more slowly than in control myofibers without drug treatment. Experiments with a virus expressing Dad1 tagged with green fluorescent protein (GFP-Dad1) showed that fluvastatin did not affect its distribution within the ER/SR network and immunofluorescence staining for GM130 did not show any marked effect on the structure of the Golgi components. Furthermore, fluvastatin did not inhibit trafficking of the chimeric transport marker VSV temperature sensitive G protein (tsG-GFP) from the ER to the Golgi. We next subjected VSV infected myofibers for pulse-chase labeling experiments and found that fluvastatin did not slow down the ER-to-Golgi trafficking or Golgi to plasma membrane trafficking of the viral glycoprotein. These studies show that fluvastatin inhibited the propagation of viral infection in skeletal myofibers but no adverse effect on the exocytic trafficking could be demonstrated. These results suggest that other effects of statins rather than inhibition of ER-to-Golgi trafficking might be behind the myotoxic effects of the statins.
机译：我们已经利用包膜病毒模型研究了氟伐他汀对离体大鼠肌纤维膜运输的影响。我们的免疫荧光研究不断显示，在未受药物治疗的对照肌纤维中，在感染水泡性口炎病毒（VSV）或A型流感病毒之前和期间用氟伐他汀治疗的肌纤维感染的传播速度较慢。用表达带有绿色荧光蛋白（GFP-Dad1）标记的Dad1的病毒进行的实验表明，氟伐他汀不影响其在ER / SR网络中的分布，GM130的免疫荧光染色未显示对高尔基组件的结构有任何显着影响。此外，氟伐他汀没有抑制嵌合转运标记物VSV温度敏感性G蛋白（tsG-GFP）从ER转运到高尔基体。接下来，我们将受VSV感染的肌纤维进行脉冲追踪标记实验，发现氟伐他汀不会减慢ER到高尔基体的运输或高尔基体对病毒糖蛋白的质膜运输的速度。这些研究表明，氟伐他汀可抑制病毒感染在骨骼肌纤维中的传播，但未显示对胞外运输的不利影响。这些结果表明，他汀类药物的其他作用而不是抑制ER向高尔基体的运输可能是他汀类药物的肌毒性作用的背后。

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