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首页> 外文期刊>Letters in Applied Microbiology >The effect of culture preservation techniques on patulin and citrinin production by Penicillium expansum Link
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The effect of culture preservation techniques on patulin and citrinin production by Penicillium expansum Link

机译：培养保存技术对青霉菌产生棒曲霉素和柠檬素的影响

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Aims: To study the influence of culture preservation methods and culture conditions on the production of the mycotoxins patulin and citrinin by Penicillium expansum . Methods and results: Ten strains of Penicillium expansum were preserved using subculture and maintenance at 4 degreesC, mineral oil, drying on silica gel and freeze-drying. Patulin and citrinin production was assessed on yeast extract sucrose agar (YES) and grape juice agar (GJ), using TLC before and after 0.5, 2-3, 6 and 12 months preservation. Citrinin was detected in all cultures for all preservation techniques on YES. The patulin profiles obtained differed with strain and culture media used. Conclusions: Citrinin production seems to be a stable character for the tested strains. There is a tendency for patulin detection with time apparently more consistent for silica gel storage and freeze-drying, especially when the strains are grown on GJ. Significance and Impact of the Study: Variability in the profiles of the mycotoxins tested seems to be more strain-specific than dependent on the preservation technique used. [References: 19]
机译：目的：研究培养保存方法和培养条件对扩展青霉产生真菌毒素棒曲霉素和柠檬素的影响。方法与结果：10株青霉菌经传代培养并保持在4℃，矿物油，硅胶干燥和冷冻干燥后保存。在保存0.5、2-3、6、12个月之前和之后，使用TLC在酵母提取物蔗糖琼脂（YES）和葡萄汁琼脂（GJ）上评估了Patulin和citrinin的产生。对于所有保存技术，在YES上都在所有培养物中检测到了Citrinin。所获得的棒曲霉素谱随所用菌株和培养基的不同而不同。结论：对于所测试的菌株，柠檬素的产生似乎是稳定的特征。巡视蛋白的检测趋势随着时间的推移在硅胶存储和冷冻干燥中表现出更加一致的趋势，尤其是当菌株在GJ上生长时。研究的意义和影响：所测试的霉菌毒素谱中的变异性似乎比依赖于所用保存技术的菌株更具特异性。 [参考：19]

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