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首页> 外文期刊>Cell biology international. >Intracellular phenotype of Mycobacterium avium enters macrophages primarily by a macropinocytosis-like mechanism and survives in a compartment that differs from that with extracellular phenotype.
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Intracellular phenotype of Mycobacterium avium enters macrophages primarily by a macropinocytosis-like mechanism and survives in a compartment that differs from that with extracellular phenotype.

机译：鸟分枝杆菌的细胞内表型主要通过巨胞饮样机制进入巨噬细胞，并在不同于细胞外表型的区室中存活。

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Mycobacterium avium uptake by human macrophages differs between the phenotypes of bacterium grown in laboratory media (extracellular growth, EG) and bacterium grown within macrophages (intracellular growth, IG). Studies in vivo have confirmed that, when spreading, pathogenic mycobacteria enter macrophages by a complement receptor 3-independent pathway, in contrast to mycobacteria uptake in vitro. M. avium, grown in macrophages (IG) for 3 or more days, invade fresh macrophages by a macropinocytosis-like mechanism, in contrast to bacteria grown in media (EG), confirmed by the inhibitory effect of wortmannin, an inhibitor of phosphoinoside-3-kinase, on the uptake of IG, but not EG, by macrophages. The IG phenotype was seen in vacuoles with lower pH than those inhabited by the EG phenotype. Incubation of macrophages with bafilomycin A1, an inhibitor of vacuole acidification, decreased the viability of intracellular IG, but not EG, phenotype, suggesting the importance of an acidic environment for the regulation of IG genes. In addition, the percentage of vacuoles that incorporate and retain LAMP-1 is smaller with EG than with IG bacteria. The formation of M. avium macropinosomes was also shown to be independent of microtubules. These data suggest that uptake of extracellular fluid is part of M. avium IG phenotype uptake by macrophages, and that the IG phenotype inhabits a slightly different vacuole than that of EG.
机译：人类巨噬细胞对鸟分枝杆菌的吸收在实验室培养基中生长的细菌（细胞外生长，EG）和在巨噬细胞中生长的细菌（细胞内生长，IG）的表型不同。体内研究证实，与体外分枝杆菌的吸收相反，病原性分枝杆菌在扩散时会通过补体受体3独立途径进入巨噬细胞。与在培养基（EG）中生长的细菌相反，在巨噬细胞（IG）中生长3天或更长时间的鸟分枝杆菌通过巨噬细胞样作用机制侵入新鲜的巨噬细胞，这一点已被渥曼青霉素（一种磷酸肌苷-抑制剂3-激酶，与巨噬细胞摄取IG而不是EG有关。在液泡中观察到IG表型，其pH低于EG表型。巨噬细胞与液泡酸化抑制剂巴氟霉素A1一起孵育会降低细胞内IG的活力，但不能降低EG表型的活力，这表明酸性环境对IG基因的调节至关重要。此外，EG合并并保留LAMP-1的液泡百分比比IG细菌小。鸟分枝杆菌大胞体的形成也被证明与微管无关。这些数据表明，细胞外液的摄取是鸟巨噬细胞IG表型被巨噬细胞摄取的一部分，并且IG表型的空泡与EG的略有不同。

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