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首页> 外文期刊>Cell biology international. >Cloning and functional characterization of a rat Daxx that functions as a corepressor for the androgen receptor.
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Cloning and functional characterization of a rat Daxx that functions as a corepressor for the androgen receptor.

机译：大鼠Daxx的克隆和功能表征，该Daxx充当雄激素受体的核心抑制剂。

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摘要

The androgen receptor (AR), a ligand-activated nuclear transcription factor, plays an important role in endocrine system development and homeostasis. AR-mediated gene transcription is modulated by a wide variety of coregulator proteins, but corepressors of AR have not been well characterized. We have isolated a rat homologue of Daxx, a possible AR repressor. The clone obtained comprised a 2196bp open reading frame, corresponding to a sequence of 731 amino acids with 80.5% homology to mouse. Basal transcription of the isolated clone was repressed in transient transcription experiments using a Gal4 reporter gene assay. Furthermore, a mammalian two-hybrid assay showed a strong interaction between Daxx and promyelocytic leukemia (PML) protein. These results indicate that the isolated clone is a rat homologue of Daxx. The rat Daxx (rDaxx) interacted with the androgen receptor (AR): in co-immunoprecipitation experiments, AR formed complexes with anti-Daxx antibody. Furthermore, overexpression of Daxx suppressed AR-mediated transcriptional activity in HeLa and CV1 cells in transient transfection experiments. Taken together, our results suggest that Daxx may function as a corepressor for the androgen receptor.
机译：雄激素受体（AR）是一种配体激活的核转录因子，在内分泌系统发育和体内平衡中起着重要作用。 AR介导的基因转录受多种共调节蛋白调节，但AR的共加压因子尚未得到很好的表征。我们已经分离出Daxx的大鼠同源物，Daxx是可能的AR阻遏物。获得的克隆包含2196bp的开放阅读框，对应于731个氨基酸的序列，与小鼠具有80.5％的同源性。分离的克隆的基础转录在瞬时转录实验中使用Gal4报告基因检测得到抑制。此外，哺乳动物的两杂交试验显示Daxx与早幼粒细胞白血病（PML）蛋白之间有很强的相互作用。这些结果表明分离的克隆是Daxx的大鼠同源物。大鼠Daxx（rDaxx）与雄激素受体（AR）相互作用：在共同免疫沉淀实验中，AR与抗Daxx抗体形成复合物。此外，在瞬时转染实验中，Daxx的过表达抑制了HeLa和CV1细胞中AR介导的转录活性。两者合计，我们的结果表明，Daxx可能充当雄激素受体的核心抑制剂。

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