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首页> 外文期刊>Cell biology international. >Characterization of the interactions between stromal and haematopoietic progenitor cells in expansion cell culture models.
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Characterization of the interactions between stromal and haematopoietic progenitor cells in expansion cell culture models.

机译：扩增细胞培养模型中基质和造血祖细胞之间相互作用的表征。

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Development of the long-term culture models of haematopoietic stem cells (HSCs) is one of the important tasks in modern biotechnology. It has been suggested that stromal presence is important for haematopoiesis in vitro and in vivo, but the question remains: whether diffusible factors produced by stromal cells are sufficient for the regeneration of primitive and definitive haematopoietic cells, or direct cell-to-cell contacts of the cultured material with underlying stromal base would be required. During present studies, influence of various feeder layers and feeder layer conditioned media on proliferative, differentiative and clonogenic activity of human AC133+ derived from human umbilical cord blood was investigated. Cell extracts for feeder layers were prepared from 4-6 weeks old human embryos and co-cultured feeder cells. Effects of the conditioned media were also determined. Culture and feeder layer media were additionally supplemented with commonly implemented factors such as GM-CSF, IL-3 and LIF. Estimation of morpho-functional properties of AC133+ cultivated suspension cultures was performed in subculture experiments using semisolid agar culture conditions. Multipotential CFU-MIX (CFU-GEMM) and unipotential progenitor cells CFU-GM, BFU-E and CFU-E were observed and analyzed. Our data suggest that haematopoiesis can be sustained for prolonged cultivation periods in the presence of feeder layer cells or conditioned media supported culture models. Prolonged support of primitive haematopoietic cells and their clonogenic capacity and functional characteristics in feeder layer positive cultures, indicates that diffusible factors are sufficient for haematopoiesis and suggests that direct cell-to-cell contacts may not be exclusively required for successful long-term in vitro haematopoiesis.
机译：造血干细胞（HSC）长期培养模型的开发是现代生物技术中的重要任务之一。有人提出基质的存在对于体外和体内的造血作用是重要的，但是问题仍然存在：基质细胞产生的可扩散因子是否足以再生原始的和定型的造血细胞，或者是否直接接触到造血细胞？需要具有基础基质的培养物。在目前的研究中，研究了各种饲养层和饲养层条件培养基对人脐带血来源的人AC133 +的增殖，分化和克隆活性的影响。用于饲养层的细胞提取物是从4-6周龄的人类胚胎和共培养的饲养细胞中制备的。还确定了条件培养基的作用。在培养和饲养层培养基中还补充了常用的因素，例如GM-CSF，IL-3和LIF。使用半固体琼脂培养条件，在亚培养实验中估算AC133 +培养的悬浮培养物的形态功能特性。观察和分析了多能CFU-MIX（CFU-GEMM）和单能祖细胞CFU-GM，BFU-E和CFU-E。我们的数据表明，在存在饲养层细胞或条件培养基支持的培养模型的情况下，造血作用可以持续较长的培养时间。在饲养层阳性培养物中对原始造血细胞及其克隆能力和功能特性的长期支持表明，扩散因子足以进行造血活动，并表明成功长期进行体外造血活动不一定仅需要直接进行细胞间接触。

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