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首页> 外文期刊>Cell biology international. >Expression of fibroblast growth factor receptor 3 by fibroblast growth factor 2 in cultured chick embryo chondrocytes.
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Expression of fibroblast growth factor receptor 3 by fibroblast growth factor 2 in cultured chick embryo chondrocytes.

机译：成纤维细胞生长因子受体3通过成纤维细胞生长因子2在培养的鸡胚软骨细胞中的表达。

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Although fibroblast growth factor 2 (FGF2) and fibroblast growth factor receptor 3 (FGFR3) both inhibit longitudinal bone growth, little is known about the relationship between FGF2 and FGFR3. Accordingly, the current study examined the expression of FGFR3 mRNA after the administration of FGF2 using cultured chondrocytes from day 17 chick embryos to evaluate the relationship between FGF2 and FGFR3. The chondrocytes were isolated from the caudal one-third portion (LS) of sterna, peripheral regions (USP) and central core regions (USC) of the cephalic portion of the sterna, and lower portion of the proximal tibial growth plate (Ti) of day 17 chick embryo. The expression of FGFR1, FGFR3, and type II and X collagen mRNA in the chondrocytes from the LS, USP, USC, and Ti was determined. FGFR1 was not expressed in the LS and USP chondrocytes, yet strongly expressed in the USC and Ti chondrocytes. With a treatment of FGF2, the expression of FGFR1 slightly increased in the USC chondrocytes and was not related with the concentration of FGF2 in the Ti chondrocytes. FGFR3 was expressed in all the chondrocyte types, yet strongly increased in the LS, USC, USP, and Ti in that order according to the concentration of FGF2. For the LS and USP chondrocytes, the expression of FGFR3 with FGF2 increased in a 4-day culture, yet decreased in a 6-day culture, whereas for the USC chondrocytes, the expression of FGFR3 mRNA with FGF2 increased in a 2-day culture, yet decreased in a 4-day culture, suggesting that the hypertrophic chondrocytes were more numerous and sensitive compared to the proliferative chondrocytes. For all the chondrocyte types, FGF2 appeared to be up-regulated to FGFR3, as the expression of FGFR3 mRNA increased with a higher concentration of FGF2 until a peak level. In conclusion, FGF2 was found to up-regulate to FGFR3 until the peak level of FGFR3 mRNA expression, while in hypertrophic chondrocytes, FGFR3 appeared to cause the differentiaton of chondrocytes, resulting in the inhibition of longitudinal bone growth after the peak level of FGFR3 mRNA expression.
机译：尽管成纤维细胞生长因子2（FGF2）和成纤维细胞生长因子受体3（FGFR3）均抑制纵向骨生长，但对FGF2和FGFR3之间的关系知之甚少。因此，当前的研究使用来自第17天鸡胚的培养的软骨细胞检查了施用FGF2后FGFR3 mRNA的表达，以评估FGF2和FGFR3之间的关系。软骨细胞从胸骨的尾部三分之一部分（LS），胸骨的头部的周围区域（USP）和中央核心区域（USC）以及胫骨近端胫骨生长板（Ti）的下部中分离第17天小鸡胚胎。确定了来自LS，USP，USC和Ti的软骨细胞中FGFR1，FGFR3以及II型和X型胶原mRNA的表达。 FGFR1在LS和USP软骨细胞中不表达，但在USC和Ti软骨细胞中强烈表达。用FGF2处理后，USC软骨细胞中FGFR1的表达略有增加，而与Ti软骨细胞中FGF2的浓度无关。 FGFR3在所有类型的软骨细胞中均有表达，但在LS，USC，USP和Ti中按FGF2的浓度依次增加。对于LS和USP软骨细胞，FGFR3和FGF2的表达在4天的培养中增加，但在6天的培养物中减少，而对于USC软骨细胞，FGFR3 mRNA和FGF2的表达在2天的培养中增加。 ，但在4天的培养中却下降了，这表明与增生的软骨细胞相比，肥大的软骨细胞数量更多且敏感。对于所有类型的软骨细胞，FGF2似乎都被上调至FGFR3，因为FGFR3 mRNA的表达随着FGF2浓度的升高而增加，直至达到峰值。总之，发现FGF2上调至FGFR3，直到FGFR3 mRNA表达达到峰值;而在肥大的软骨细胞中，FGFR3似乎引起软骨细胞的分化，从而在FGFR3 mRNA达到峰值后抑制了纵向骨生长。表达。

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