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首页> 外文期刊>Cell biology international. >Senescent phenotype achieved in vitro is indistinguishable, with the exception of Bcl-2 content, from that attained during the in vivo aging process.
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Senescent phenotype achieved in vitro is indistinguishable, with the exception of Bcl-2 content, from that attained during the in vivo aging process.

机译：除Bcl-2含量外，在体外获得的衰老表型与在体内衰老过程中获得的表型没有区别。

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摘要

Senescent phenotype can be attained by diverse agents, thus suggesting that there might be molecular differences between the senescence achieved in vivo and the senescence-like state attained in vitro under culture conditions. In this study we compare the senescent phenotype reached by cells derived from young animals when cultured in vitro with the one associated with the in vivo aging process. Several in vitro senescence parameters, including MTT reduction, proliferation rate, DNA synthesis, SA-beta-gal staining, and both in vivo and in vitro Bcl-2 content, were determined. Alterations in DNA electrophoretic mobility were evaluated to test differences in bulk chromatin structure. Our results indicate that although it is possible to achieve a senescent phenotype with cells derived from young animals aged in culture, this phenotype differs from the one observed in older animals, due to lack of in vivo damage inducers to which cells are being exposed during natural aging.
机译：可以通过多种药物获得衰老表型，因此表明在培养条件下，体内获得的衰老与体外获得的衰老样状态之间可能存在分子差异。在这项研究中，我们比较了与体外衰老过程相关的体外培养时，来自幼小动物的细胞达到的衰老表型。确定了几个体外衰老参数，包括MTT降低，增殖速率，DNA合成，SA-β-gal染色以及体内和体外Bcl-2含量。评估DNA电泳迁移率的变化以测试整体染色质结构的差异。我们的结果表明，尽管可以用培养中的年幼动物的细胞实现衰老表型，但由于缺乏在自然过程中暴露于细胞的体内损伤诱导剂，因此该表型与在年长动物中观察到的表型不同。老化。

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