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首页> 外文期刊>Cell biology international. >Unique membrane-interacting properties of the immunostimulatory cationic peptide KLKL(5)KLK (KLK).
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Unique membrane-interacting properties of the immunostimulatory cationic peptide KLKL(5)KLK (KLK).

机译：免疫刺激性阳离子肽KLKL（5）KLK（KLK）的独特膜相互作用特性。

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摘要

We have monitored the effects of KLKL(5)KLK (KLK), a derivative of a natural cationic antimicrobial peptide (CAP) on isolated membrane vesicles, and investigated the partition of the peptide within these structures. KLK readily interacted with fluorescent dyes entrapped in the vesicles without apparent pore formation. Fractionation of vesicles revealed KLK predominantly in the membrane. Peptide-treated vesicles appeared with generally disorganized bilayers. While KLK showed no effect on osmotic resistance of human erythrocytes, dramatic decrease in core and surface membrane fluidity was observed in peptide-treated erythrocyte ghosts as measured by fluorescence anisotropy. Finally, CD spectroscopy revealed lipid-induced random coil to beta-sheet and beta-sheet to alpha-helix conformational transitions of KLK. Together with the oligonucleotide oligo-d(IC)(13) [ODN1a], KLK functions as a novel adjuvant, termed IC31. Among other immunological effects, KLK appears to facilitate the uptake and delivery of ODN1a into cellular compartments, but the nature of KLK's interaction with the cell surface and other membrane-bordered compartments remains unknown. Our results suggest a profound membrane interacting property of KLK that might contribute to the immunostimulatory activities of IC31.
机译：我们已经监测了KLKL（5）KLK（KLK），一种天然阳离子抗微生物肽（CAP）的衍生物对分离的膜囊泡的影响，并研究了这些结构中肽的分配。 KLK容易与截留在囊泡中的荧光染料相互作用而没有明显的孔形成。囊泡的分级显示KLK主要在膜中。肽处理的囊泡通常具有混乱的双层结构。虽然KLK对人红细胞的渗透阻力没有影响，但通过荧光各向异性测量，在肽处理的红细胞鬼影中观察到核心和表面膜流动性显着降低。最后，CD光谱揭示了脂质诱导的KLK的无规卷曲到β-折叠和β-折叠到α-螺旋构象转变。 KLK与寡核苷酸oligo-d（IC）（13）[ODN1a]一起用作新型佐剂，称为IC31。在其他免疫学作用中，KLK似乎促进了ODN1a的吸收和向细胞区室的传递，但是KLK与细胞表面和其他跨膜区室的相互作用的性质仍然未知。我们的结果表明，KLK具有深厚的膜相互作用特性，可能有助于IC31的免疫刺激活性。

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