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首页> 外文期刊>Cell biology international. >Subcellular distribution of spermidine/spermine N1-acetyltransferase
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Subcellular distribution of spermidine/spermine N1-acetyltransferase

机译：亚精胺/亚精胺N1-乙酰基转移酶的亚细胞分布

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摘要

The subcellular distribution of the polyamine catabolic enzyme spermidine/spermine N1-acetyltransferase (SSAT) was studied in L56Br-C1 cells treated with 10 μM N1,N11-diethylnorspermine (DENSPM) for 24 h. Cells were fractioned into three subcellular fractions. A particulate fraction containing the mitochondria was denoted as the mitochondrial fraction. After DENSPM treatment, an increase in SSAT activity was mainly found in the mitochondrial fraction. Western blot analysis showed an increased level of the SSAT protein in the mitochondrial fraction compared to the cytosolic fraction. Immunofluorescence microscopy and immunogold labeling transmission electron microscopy also showed a mitochondrial association of SSAT. Transmission electron microscopy revealed that the endoplasmic reticulum was devoid of ribosomes in DENSPM-treated cells, in contrast to control cells that contained ample ribosomes. An increased SSAT activity in connection with the mitochondria may be part of the mechanism of DENSPM-induced apoptosis.
机译：研究了多胺分解代谢酶亚精胺/亚精胺N1-乙酰基转移酶（SSAT）在亚铁分布中的作用，该细胞在经10μMN1，N11-二乙基去甲精胺（DENSPM）处理24 h的L56Br-C1细胞中进行了研究。将细胞分为三个亚细胞部分。包含线粒体的颗粒级分表示为线粒体级分。 DENSPM处理后，SSAT活性增加主要在线粒体部分。蛋白质印迹分析显示，与胞质组分相比，线粒体组分中SSAT蛋白的含量增加。免疫荧光显微镜和免疫金标记透射电子显微镜也显示了SSAT的线粒体缔合。透射电子显微镜显示，与包含充足核糖体的对照细胞相比，DENSPM处理的细胞内质网中没有核糖体。与线粒体有关的SSAT活性增加可能是DENSPM诱导凋亡的机制的一部分。

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