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首页> 外文期刊>Cell biology international. >Detection of intranucleolar chromatin using an ultrastructural immunolabelling technique.
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Detection of intranucleolar chromatin using an ultrastructural immunolabelling technique.

机译：使用超微结构免疫标记技术检测核仁内染色质。

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摘要

The intranucleolar chromatin has been detected by feeding HeLa cells with bromodeoxyuridine and then using an immunocytochemical technique for its detection at the ultrastructural level. Labelling was mainly observed on the dense fibrillar component (DFC), which surrounds the fibrillar centres (FC). Labelling could also be seen on the network within the granular component (GC) of the nucleoli. Ring shaped nucleoli with large FCs showed labelling, in addition, on the FCs. Nucleoli from spread preparations of cells treated with hypotonic salt solution for different periods revealed a nucleolonemal network, consisting of units comparable to rDNA transcriptional units in length and associated with tufts of fibrils and granules. Thread-like axes could be detected running through the units and in between two units. Chromatin could also be identified within these units by the immunolabelling technique. The observations strongly indicate that these units are identical with the rDNA transcriptional units. A dynamic nature of the nucleolar chromatin and nucleolar organization has been discussed. Copyright 1998 Academic Press.
机译：核内染色质已通过向HeLa细胞中加入溴脱氧尿苷进行检测，然后使用免疫细胞化学技术对其超微结构水平进行检测。标记主要在围绕原纤维中心（FC）的致密​​原纤维成分（DFC）上观察到。在核仁的颗粒成分（GC）内的网络上也可以看到标记。此外，具有较大FC的环状核仁在FC上显示标记。低渗盐溶液处理细胞在不同时期的分散制剂中的核仁显示出核仁网络，该核仁网络的长度与rDNA转录单位相当，并且与原纤维簇和颗粒簇相关。可以检测到贯穿两个单元的螺纹状轴。染色质也可以通过免疫标记技术在这些单位内鉴定。观察结果强烈表明这些单位与rDNA转录单位相同。已经讨论了核仁染色质和核仁组织的动态性质。版权所有1998学术出版社。

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