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Cytokinesis in cells undergoing mitosis without genome replication.

机译:没有基因组复制的有丝分裂细胞的细胞分裂。

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Chinese hamster ovary cells can be forced to enter mitosis without prior DNA replication by treatment with hydroxyurea and caffeine. Cells treated in this way assemble a spindle that functions normally except that it does not accomplish anaphase spindle elongation (anaphase B). The chromatin detaches from the unreplicated kinetochores, which fragment, but establish microtubule attachments and migrate to the metaphase plate. Partitioning of the kinetochore fragments ensues on the normal schedule. Typical midbodies and cleavage furrows are established and daughter cells of equal size are produced. These results imply that intact chromosomes are not necessary for correct cleavage furrow placement but that kinetochores might be. Further, it is clear that cleavage furrow placement does not depend on anaphase spindle elongation. Copyright 1999 Academic Press.
机译:通过用羟基脲和咖啡因处理,中国仓鼠卵巢细胞可被迫进入有丝分裂而无需事先复制DNA。用这种方法处理过的细胞会组装一个正常运行的纺锤,但它不能完成后期纺锤体伸长(后期B)。染色质与未复制的动植物分离,后者分裂,但建立微管附着并迁移至中期板。线粒体片段的分区按正常时间表进行。建立了典型的中体和裂沟,并产生了大小相等的子细胞。这些结果表明完整的染色体对于正确的切割沟位不是必需的,但是动植物可能是完整的。此外,很明显,劈沟的放置不依赖于后期纺锤体的伸长。版权所有1999,学术出版社。

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