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首页> 外文期刊>Cell biology international. >Airway epithelial cell-derived insulin-like growth factor-1 triggers skewed CD8~+ T cell polarization
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Airway epithelial cell-derived insulin-like growth factor-1 triggers skewed CD8~+ T cell polarization

机译：气道上皮细胞衍生的胰岛素样生长因子-1触发偏斜的CD8〜+ T细胞极化

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摘要

Skewed CD8~+ T cell responses are important in airway inflammation. This study investigates the role of the airway epithelial cell-derived insulin-like growth factor 1 (IGF1) in contributing to CD8~+ T cell polarization. Expression of IGF1 in the airway epithelial cell line, RPMI2650 cells, was assessed by quantitative real time RT-PCR and Western blotting. The role of IGF1 in regulating CD8~+ T cell activation was observed by coculture of mite allergen-primed RPMI2650 cells and naive CD8~+ T cells. CD8~+ T cell polarization was assessed by the carboxyfluorescein succinimidyl ester-dilution assay and the determination of cytotoxic cytokine levels in the culture medium. Exposure to mite allergen, Der p1, increased the expression of IGF1 by RPMI2650 cells. The epithelial cell-derived IGF1 prevented the activation-induced cell death by inducing the p53 gene hypermethylation. Mite allergen-primed RPMI2650 cells induced an antigen-specific CD8~+ T cell polarization. We conclude that mite allergens induce airway epithelial cell line, RPMI2650 cells, to produce IGF1; the latter contributes to antigen-specific CD8~+ T cell polarization.
机译：偏斜的CD8〜+ T细胞反应在气道炎症中很重要。本研究探讨了气道上皮细胞衍生的胰岛素样生长因子1（IGF1）在CD8〜+ T细胞极化中的作用。通过定量实时RT-PCR和Western印迹评估IGF1在气道上皮细胞系RPMI2650细胞中的表达。通过螨过敏原引发的RPMI2650细胞和幼稚CD8 + T细胞的共培养观察到IGF1在调节CD8 + T细胞活化中的作用。通过羧基荧光素琥珀酰亚胺酯稀释法和培养基中细胞毒性细胞因子水平的测定来评估CD8 + T细胞的极化。暴露于螨过敏原Der p1后，RPMI2650细胞增加了IGF1的表达。上皮细胞衍生的IGF1通过诱导p53基因高度甲基化来防止激活诱导的细胞死亡。螨过敏原引发的RPMI2650细胞诱导了抗原特异性CD8〜+ T细胞极化。我们得出的结论是，螨过敏原会诱导气道上皮细胞系RPMI2650细胞产生IGF1。后者有助于抗原特异性CD8〜+ T细胞极化。

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