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首页> 外文期刊>Cell biology international. >The Tetrahymena homolog of bacterial and mammalian 4-hydroxyphenylpyruvate dioxygenases localizes to membranes of the endoplasmic reticulum.
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The Tetrahymena homolog of bacterial and mammalian 4-hydroxyphenylpyruvate dioxygenases localizes to membranes of the endoplasmic reticulum.

机译：细菌和哺乳动物的4-羟苯基丙酮酸双加氧酶的四膜虫同系物定位于内质网的膜。

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摘要

The expression and intracellular localization of the Tetrahymena homolog of 4-hydroxyphenylpyruvate dioxygenase (HPPD) were investigated in wild-type Tetrahymena thermophila strain B1868 VII and the mutant strains IIG8, defective in food vacuole formation, MS-1, blocked in secretion of lysosomal enzymes, and SB 281, defective in mucocyst maturation. Immunoelectron microscopy and confocal laser scanning microscopy demonstrated that Tetrahymena HPPD primarily localized to membranes of the endoplasmic reticulum. In addition, Tetrahymena HPPD was detected in association with membranes of the Golgi apparatus, and transport vesicles in exponentially growing wild-type and mutant strains. In starved cells, Tetrahymena HPPD localized exclusively to membranes of small vesicles. Since no de novo synthesis of Tetrahymena HPPD takes place in cells starved for more than 30 min, these results suggest that there is a flow of Tetrahymena HPPD from the endoplasmic reticulum to small vesicles, possibly via the Golgi apparatus, and that Tetrahymena HPPD contains a signal for vesicle membrane retrieval or retention. Copyright 1999 Academic Press.
机译：研究了4-羟基苯基丙酮酸双加氧酶（HPPD）的四膜虫膜同源物在野生型四膜膜嗜热菌菌株B1868 VII中的表达和细胞内定位，以及在食物液泡形成方面存在缺陷的突变株IIG8，MS-1，溶酶体酶的分泌受到阻滞。和SB 281，在黏液囊成熟方面存在缺陷。免疫电子显微镜和共聚焦激光扫描显微镜证明，膜膜内膜四分菌HPPD主要位于内质网膜上。此外，四膜膜HPPD被发现与高尔基体的膜相关联，并在成倍增长的野生型和突变菌株中运输囊泡。在饥饿的细胞中，四膜虫HPPD仅定位于小囊泡的膜。由于饥饿超过30分钟的细胞中未发生四膜虫HPPD的从头合成，因此这些结果表明四膜虫HPPD可能通过高尔基体从内质网流向小囊泡，并且四膜虫HPPD含有一种囊泡膜恢复或保留的信号。版权所有1999，学术出版社。

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