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首页> 外文期刊>Cell and Tissue Research >Aromatase expression in cultured fetal sheep astrocytes after nitrosative/oxidative damage.
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Aromatase expression in cultured fetal sheep astrocytes after nitrosative/oxidative damage.

机译：亚硝基/氧化损伤后培养的胎羊星形胶质细胞中芳香酶的表达。

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摘要

Aromatase, the enzyme converting androgens into estrogens, is involved in many brain processes such as neural differentiation and plasticity or the prevention of cell death. We have previously observed an increase in aromatase immunoreactivity in sheep neurons exposed in vitro to the oxidant 3-nitro-L: -tyrosine. However, little is known regarding the way that sheep astrocytes cope with nitrosative stress, a condition occurring in sheep in the pathogenesis of neurodegenerative disorders such as scrapie and Maedi-Visna. Our aim has been to evaluate the effects of 3-nitro-L-tyrosine on astrocyte primary cultures from 90-day-old fetal sheep brain. Living cells were observed and characterized by immunofluorescence with a GFAP antibody, which indicated that the majority of the cells were astrocytes. A viability assay was performed on both untreated and treated cells. Reverse transcription with the polymerase chain reaction was undertaken to monitor time- and dose-dependent variations in aromatase gene expression. Stressed astrocytes showed signs of deterioration, were reduced in number, and appeared round with few short processes; the cell death rate was approximately 30%. Aromatase expression was detected starting from a 24-h exposure to 1 mM 3-nitro-L-tyrosine and reached the highest levels at 72 h. Thus, oxidative damage probably results in the local production of neuroprotective estradiol by reactive astrocytes via the aromatization of testosterone.
机译：芳香酶是一种将雄激素转化为雌激素的酶，它参与许多大脑过程，例如神经分化和可塑性或预防细胞死亡。我们以前已经观察到体外暴露于氧化剂3-硝基-L：-酪氨酸的绵羊神经元芳香酶的免疫反应性增加。然而，关于羊星形胶质细胞应对亚硝化应激的方式所知甚少，亚硝化应激是绵羊在神经退行性疾病如瘙痒病和前田维斯纳病的发病机理中所发生的疾病。我们的目的是评估3-硝基-L-酪氨酸对90天大的胎羊脑星形胶质细胞原代培养的影响。观察到活细胞并用GFAP抗体通过免疫荧光进行了表征，这表明大多数细胞是星形胶质细胞。对未处理和处理的细胞均进行了活力测定。用聚合酶链反应进行逆转录以监测芳香酶基因表达的时间和剂量依赖性变化。应激的星形胶质细胞显示出退化的迹象，数量减少，并呈圆形，几乎没有短过程。细胞死亡率约为30％。从暴露于1 mM 3-硝基-L-酪氨酸的24小时开始检测到芳香酶表达，并在72 h达到最高水平。因此，氧化损伤可能导致反应性星形胶质细胞通过睾丸激素的芳香化而局部产生神经保护性雌二醇。

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