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首页> 外文期刊>Cell and Tissue Research >Comparative effect of FGF2, synthetic peptides 1-28 N-POMC and ACTH on proliferation in rat adrenal cell primary cultures.
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Comparative effect of FGF2, synthetic peptides 1-28 N-POMC and ACTH on proliferation in rat adrenal cell primary cultures.

机译：FGF2，合成肽1-28 N-POMC和ACTH对大鼠肾上腺细胞原代培养物增殖的比较作用。

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摘要

There is evidence that pro-opiomelanocortin (POMC)-derived peptides other than adrenocorticotropic hormone (ACTH) have a role in adrenal cell proliferation. We compared the activity of synthetic rat N-terminal POMC fragment 1-28 with disulfide bridges (N-POMC(w)) and without disulfide bridges (N-POMC(w/o)), with the activity of fibroblast growth factor (FGF2), a widely studied adrenal growth factor, and ACTH, in well-characterized pure cultures of both isolated adrenal Glomerulosa (G) and Fasciculata/Reticularis (F/R) cells. Three days of FGF2-treatment had a proliferative effect similar to serum, and synthetic peptide N-POMC(w) induced proliferation more efficiently than N-POMC(w/o). Moreover, both induced proliferation via the ERK1/2 pathway. In contrast, sustained ACTH treatment decreased proliferation and viability through apoptosis induction, but not necrosis, and independently of PKA and PKC pathways. Further elucidation of 1-28 POMC signal transduction is of interest, and primary cultures of adrenal cells were found to be useful for examining the trophic activity of this peptide.
机译：有证据表明，除了促肾上腺皮质激素（ACTH）以外，源自前阿维黑素皮质素（POMC）的肽在肾上腺细胞增殖中也起作用。我们比较了具有二硫键（N-POMC（w））和无二硫键（N-POMC（w / o））的合成大鼠N末端POMC片段1-28的活性与成纤维细胞生长因子（FGF2）的活性），在分离的肾上腺小球（G）和Fasciculata / Reticularis（F / R）细胞的特征明确的纯培养物中，广泛研究的肾上腺生长因子和ACTH。三天的FGF2处理具有类似于血清的增殖作用，合成肽N-POMC（w）比N-POMC（w / o）更有效地诱导增殖。此外，两者均通过ERK1 / 2途径诱导增殖。相反，持续的ACTH治疗通过凋亡诱导而不是坏死来降低增殖和活力，并且独立于PKA和PKC途径。 1-28 POMC信号转导的进一步阐明是令人感兴趣的，并且发现肾上腺细胞的原代培养可用于检查该肽的营养活性。

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