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首页> 外文期刊>Cell and Tissue Research >Clustering of Kir4.1 at specialized compartments of the lateral membrane in ependymal cells of rat brain
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Clustering of Kir4.1 at specialized compartments of the lateral membrane in ependymal cells of rat brain

机译：Kir4.1聚集在大鼠大脑室管膜细胞侧膜的特殊隔室中

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Brain ependymal cells, which form an epithelial layer covering the cerebral ventricles, have been shown to play a role in the regulation of cerebrospinal and interstitial fluids. The machinery underlying this, however, remains largely unknown. Here, we report the specific localization of an inwardly rectifying K+ channel, Kir4.1, on the ependymal cell membrane suggesting involvement of the channel in this function. Immunohistochemical study with confocal microscopy identified Kir4.1 labeling on the lateral but not apical membrane of ependymal cells. Ultrastructural analysis revealed that Kir4.1-immunogold particles were specifically localized and clustered on adjacent membranes at puncta adherens type junctions, whereas an aquaporin water channel, AQP4, that was also detected on the lateral membrane only occurred at components other than adherens junctions. Therefore, in ependymal cells, Kir4.1 and AQP4 are partitioned into distinct membrane compartments that might respectively transport either K+ or water. Kir4.1 was also expressed in a specialized form of ependymal cell, namely the tanycyte, being abundant in tanycyte processes wrapping neuropils and blood vessels. These specific localizations suggest that Kir4.1 mediates intercellular K+ exchange between ependymal cells and also K+-buffering transport via tanycytes that can interconnect neurons and vessels/ventricles. We propose that ependymal cells and tanycytes differentially operate Kir4.1 and AQP4 actively to control the property of fluids at local areas in the brain.
机译：已经显示形成覆盖脑室的上皮层的脑室管膜细胞在调节脑脊液和间质液中起作用。然而，其背后的机制仍然未知。在这里，我们报告内质网膜细胞膜上向内整流的K +通道，Kir4.1的具体定位，表明该通道参与此功能。共聚焦显微镜的免疫组织化学研究确定了室管膜细胞的外侧膜但不是顶膜上的Kir4.1标记。超微结构分析显示，Kir4.1-免疫金颗粒特异性定位并聚集在点状粘附型连接处的相邻膜上，而在侧膜上也检测到的水通道水通道AQP4仅发生在粘附连接以外的组件上。因此，在室管膜细胞中，Kir4.1和AQP4分为不同的膜区室，分别可转运K +或水。 Kir4.1还以特异的室管膜细胞形式表达，即单核细胞，在包裹神经纤维和血管的单核细胞过程中含量很高。这些特定的位置表明，Kir4.1介导室管膜细胞之间的细胞间K +交换，还通过单核细胞介导K +缓冲转运，单核细胞可以互连神经元和血管/心室。我们建议，室管膜细胞和单核细胞有区别地运作Kir4.1和AQP4积极控制大脑局部区域的液体的属性。

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