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首页> 外文期刊>Cell biology international. >In vitro behaviour of human osteoblasts on dentin and bone.
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In vitro behaviour of human osteoblasts on dentin and bone.

机译：人成骨细胞在牙本质和骨骼上的体外行为。

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This investigation studied how the behaviour of isolated osteoblasts on standard tissue culture polystyrene compared with cells cultured on cut surfaces of dentin, a natural calcified material. Cellular attachment, viability and growth were monitored in parallel cultures of human osteosarcoma cell lines (MG63, HOS TE85, SaOS-2) and primary human osteoblast-like cells (HOBs). Culture plastic was either left untreated or roughened with abrasive paper of various grit sizes (4000-1200 grit) in order to obtain a level of roughness comparable to that of the dentin slices. Cell counting and intracellular BCECF staining showed that after an initial incubation of 2 h, the primary cells attached and spread out more quickly on the different substrates than the three cell lines. The primary cells also showed a stronger mitochondrial staining and viability on dentin. During subsequent culture morphological differences appeared with the cells on dentin displaying more cellular extensions. All three cell lines proliferated more slowly on dentin than on plastic. In contrast, the primary HOBs were not significantly affected in their growth by the different substrates. Total and specific alkaline phosphatase (AP) activity of the cell lines was not significantly affected by the different substrates after short-term adhesion, but it was increased for the primary cells on the dentin. However, after 2-3 days of culture, AP was decreased on the dentin slices for both the cell lines and primary HOBs. Plasma treatment of the roughened plastic did not alter cellular viability or AP activity, suggesting that grinding of the surface did not affect the property of the culture plastic to support cell attachment and growth. In conclusion, the results show that not only do osteoblastic cells behave differently on a natural calcified substrate surface than on standard culture plastic, but also that differences were evident between the various cell types, in particular the primary HOB versus the continuous cell lines.
机译：这项研究研究了与在自然钙化材料牙本质切面上培养的细胞相比，在标准组织培养物聚苯乙烯上分离的成骨细胞的行为。在人骨肉瘤细胞系（MG63，HOS TE85，SaOS-2）和原代人成骨细胞样细胞（HOB）的平行培养物中监测细胞附着，活力和生长。将培养塑料未经处理或用各种粒度（4000-1200粒度）的砂纸进行粗糙处理，以获得与牙本质切片相当的粗糙度水平。细胞计数和细胞内BCECF染色显示，最初孵育2小时后，原代细胞比三种细胞系附着并在不同底物上扩散得更快。原代细胞还显示出更强的线粒体染色和对牙本质的活力。在随后的培养过程中，出现了形态学差异，牙本质上的细胞显示出更多的细胞延伸。所有三种细胞系在牙本质上的增殖均比在塑料上的增殖慢。相反，初级HOBs的生长不受不同底物的影响。短期粘附后，不同底物对细胞系的总和特定碱性磷酸酶（AP）活性没有显着影响，但对于牙本质上的原代细胞，其活性增加了。然而，培养2-3天后，细胞系和原代HOBs在牙本质切片上的AP均降低。对粗糙塑料的等离子体处理不会改变细胞活力或AP活性，这表明表面的研磨不会影响培养塑料支持细胞附着和生长的特性。总之，结果表明，不仅成骨细胞在天然钙化基质表面上的行为不同于在标准培养塑料上的行为，而且在各种细胞类型之间，尤其是原代HOB与连续细胞系之间也存在明显差异。

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