• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Glyceraldehyde-3-phosphate dehydrogenase associates with actin filaments in serum deprived NIH 3T3 cells only.
【24h】

Glyceraldehyde-3-phosphate dehydrogenase associates with actin filaments in serum deprived NIH 3T3 cells only.

机译：甘油醛-3-磷酸脱氢酶仅与血清缺乏的NIH 3T3细胞中的肌动蛋白丝缔合。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The in vitro interaction between the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and cytoskeletal elements is well documented. To verify this association within cells, the intracellular distribution of GAPDH under various metabolic conditions has been investigated in immunostained cells or cells expressing GAPDH as a GFP fusion protein. GAPDH was homogeneously distributed in the cytoplasm and no interaction of GAPDH with cytoskeletal elements, neither with microfilaments nor microtubules or intermediate filaments, was detectable. In living cells expressing GFP-GAPDH, stress fibres were excluded from the fluorescence. In contrast to proliferating cells, the cytoplasmic GAPDH of serum-depleted cells was not homogeneously distributed, but colocalised with stress fibres. The mechanism for stimulating this actin-binding affinity was independent of the NO-signalling pathway. The results support the idea of a specialised function for the interaction of GAPDH and cytoskeletal elements, rather than a general function, as e.g. microcompartmentalization of glycolytic enzymes.
机译：糖酵解酶-3-磷酸甘油醛脱氢酶（GAPDH）与细胞骨架元素之间的体外相互作用已得到充分证明。为了证实细胞内的这种联系，已经在免疫染色的细胞或表达GAPDH为GFP融合蛋白的细胞中研究了GAPDH在各种代谢条件下的细胞内分布。 GAPDH均匀地分布在细胞质中，没有检测到GAPDH与细胞骨架成分的相互作用，无论是微丝还是微管或中间丝。在表达GFP-GAPDH的活细胞中，应力纤维被排除在荧光之外。与增殖细胞相反，贫血血清细胞的细胞质GAPDH不是均匀分布，而是与应力纤维共存。刺激这种肌动蛋白结合亲和力的机制独立于NO信号通路。结果支持GAPDH和细胞骨架元件相互作用的专门功能的想法，而不是一般的功能，例如。糖酵解酶的微区室化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号